Fc-IgM and Fc-IgG receptors on human circulating B lymphocytes.
Fc-IgM and Fc-IgG receptors on human circulating B lymphocytes.
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人循环 B 淋巴细胞上的 Fc-IgM 和 Fc-IgG 受体。
DOI：
10.4049/jimmunol.121.3.887
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发表时间：
1978
影响因子：
4.4
通讯作者：
Samuel Broder
中科院分区：
文献类型：
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作者：
Werner J. Pichler;Samuel Broder
用玫瑰花环技术对正常人外周血B细胞进行了浓缩，平均表面免疫球蛋白阳性率为93.5%。我们用包被免疫球蛋白抗体(Ea-Ig G)和免疫球蛋白M(Ea-Ig M)的牛红细胞研究了这些纯化的B细胞Fc-Ig G和Fc-Ig M受体的表达。用这种灵敏的玫瑰花环技术检测到81%~94%(平均88.67%)的Fc-Ig G和79%~97%(平均87.5%)的Fc-IgM受体阳性细胞。
37℃培养24、48、72小时后，Fc-Ig G受体表达增强。胰酶(0.25%)去除Fc-IgM受体，但不去除Fc-Ig G受体。然而，经胰酶消化的细胞进一步培养后，Fc-IgM受体在相同比例甚至更高比例的细胞上重新表达。
表达δ或γ重链的表面免疫球蛋白的单克隆性B细胞(慢性淋巴细胞性白血病细胞)也检测到Fc-Ig M受体。正常外周血B细胞中高比例的Fc-IgM阳性细胞，再加上Fc-IgM受体并不局限于具有特定表面免疫球蛋白的单克隆性B细胞，提示Fc-IgM受体可能存在于基本上所有的B细胞上。此外，我们的结果表明，大多数人B细胞同时表达Fc-IgM和Fc-Ig G受体。
Peripheral blood B cells from normal humans were enriched with rosette techniques (mean 93.5% surface immunoglobulin positive). We studied these purified B cells for the expression of Fc-IgG and Fc-IgM receptors by using bovine red blood cells coated with IgG (EA-IgG) or IgM (EA-IgM). Between 81 and 94% (mean 88.67%) Fc-IgG and between 79 and 97% (mean 87.5%) Fc-IgM receptor positive cells were found by using this sensitive rosette technique.
The Fc-IgG receptor values had a tendency to decrease in culutre at 37°C. In contrast, cultivation at 37°C for 24, 48, and even 72 hr enhanced Fc-IgM receptor expression. Trypsinization (0.25%) removed Fc-IgM receptors, but not Fc-IgG receptors. However, after further cultivation of the trypsinized cells, Fc-IgM receptors were re-expressed on the same or even greater percentages of cells.
Fc-IgM receptors were also detected on monoclonal B cells (chronic lymphatic leukemia cells) expressing surface immunoglobulins with either δ or γ heavy chains exclusively. The high percentage of Fc-IgM positive cells in normal peripheral blood B cells, coupled with the observation that the Fc-IgM receptors are not confined to monoclonal B cells with particular classes of surface immunoglobulins, suggests that the Fc-IgM receptor may be present on essentially all B cells. Moreover, our results suggest that most human B cells expressed both Fc-IgM and Fc-IgG receptors simultaneously.