HOMOLOGY BETWEEN IRE-BP, A REGULATORY RNA-BINDING PROTEIN, ACONITASE, AND ISOPROPYLMALEATE ISOMERASE
HOMOLOGY BETWEEN IRE-BP, A REGULATORY RNA-BINDING PROTEIN, ACONITASE, AND ISOPROPYLMALEATE ISOMERASE
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DOI：
10.1093/nar/19.8.1739
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发表时间：
1991-04-25
影响因子：
14.9
通讯作者：
ARGOS, P
中科院分区：
文献类型：
--
作者：
HENTZE, MW;ARGOS, P
铁响应元件（IREs）是作为IRE-BP特异性结合位点的RNA调控元件。IREs和IRE-BP之间的相互作用诱导铁蛋白mRNA翻译的抑制和转铁蛋白受体mRNA的稳定。我们描述了IRE-BP和两种已知的异构酶，顺乌头酸酶和苹果酸异丙酯（IPM）异构酶之间的广泛的氨基酸序列同源性的鉴定。我们讨论了这一观察结果的影响方面的IRE-BP的结构/功能关系。调节RNA结合蛋白和参与中间代谢的两种酶之间的结构保守性提供了生物结构中功能灵活性的令人惊讶的例子。
Iron-responsive elements (IREs) are regulatory RNA elements which serve as specific binding sites for the IRE-binding protein (IRE-BP). Interaction between IREs and IRE-BP induces repression of ferritin mRNA translation and transferrin receptor mRNA stabilization. We describe the identification of extensive amino acid sequence homology between IRE-BP and two known isomerases, aconitase and isopropylmalate (IPM) isomerase. We discuss the implications of this observation with regard to structure/function relationships of IRE-BP. The structural conservation between a regulatory RNA-binding protein and two enzymes involved in intermediary metabolism provides a surprising example of the functional flexibility in biological structures.