Effects of copper and zinc oxide nanoparticles on German cockroach development, indoxacarb resistance, and bacterial load.
Effects of copper and zinc oxide nanoparticles on German cockroach development, indoxacarb resistance, and bacterial load.
复制标题
铜和氧化锌纳米颗粒对德国小蠊发育、茚虫威抗性和细菌负荷的影响。
DOI：
10.1002/ps.7472
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发表时间：
2023
影响因子：
4.1
通讯作者：
Pietri,JoseE
中科院分区：
文献类型：
--
作者：
Zha,Chen;Turner,Matthew;Ray,Ritesh;Liang,Dangsheng;Pietri,JoseE
德国蟑螂，德国小蠊，是一种普遍存在的医学上重要的城市害虫。目前，在全球范围内，B.德国的控制工作十分复杂，因此需要改进工具。我们以前报道过，通过口服抗微生物多西环素破坏肠道微生物群，降低了茚虫威抗性田间菌株的抗性，也延迟了若虫发育，降低了成虫繁殖力。然而，多西环素在该领域用于蟑螂控制是不切实际的。在这里，我们试图确定两种具有已知抗菌特性的金属纳米颗粒，铜（Cu）和氧化锌（ZnO），是否与强力霉素对B的生理学具有相似的作用。我们发现，膳食暴露于0.1%的铜纳米颗粒，而不是氧化锌，显着延迟发展成成人的恶性肿瘤。然而，无论是纳米粒子改变了女性的生殖力，和ZnO令人惊讶地增加了抗性茚虫威在耐药现场应变，与强力霉素。半定量聚合酶链反应（qPCR）进一步显示，长时间（14天）饮食暴露于低浓度的Cu或ZnO纳米颗粒，（0.1%）并没有减少细菌微生物群的负荷，这表明了它们所观察到的效果背后的替代机制。我们的研究结果表明，摄入铜纳米颗粒可以通过一种不涉及减少细菌微生物群总负荷的未确定机制影响德国小蠊的发育。因此，铜可能有一些应用在蟑螂控制作为这种活动的结果，但在评估潜在的蟑螂控制的纳米粒子时，应考虑对杀虫剂抗性的拮抗作用。© 2023化学工业学会。
BACKGROUNDThe German cockroach,Blattella germanica, is a ubiquitous and medically significant urban pest. The ongoing development of insecticide resistance in global populations ofB. germanicahas complicated control efforts and created a need for improved tools. We previously reported that disruption of the gut microbiota by oral administration of the antimicrobial doxycycline reduced resistance in an indoxacarb resistant field strain and also delayed nymphal development and reduced adult fecundity. However, the application of doxycycline for cockroach control in the field is impractical. Here, we sought to determine whether two metal nanoparticles with known antimicrobial properties, copper (Cu) and zinc oxide (ZnO), have similar effects to doxycycline on the physiology ofB. germanicaand could provide more practical alternatives for control.RESULTSWe found that dietary exposure to 0.1% Cu nanoparticles, but not ZnO, significantly delays the development of nymphs into adults. However, neither of the nanoparticles altered the fecundity of females, and ZnO surprisingly increased resistance to indoxacarb in a resistant field strain, in contrast to doxycycline. Semi‐quantitative polymerase chain reaction (qPCR) further revealed that prolonged dietary exposure (14 days) to Cu or ZnO nanoparticles at the low concentration readily consumed by cockroaches (0.1%) does not reduce the load of the bacterial microbiota, suggesting alternative mechanisms behind their observed effects.CONCLUSIONSTogether, our results indicate that ingestion of Cu nanoparticles can impact German cockroach development through an undetermined mechanism that does not involve reducing the overall load of the bacterial microbiota. Therefore, Cu may have some applications in cockroach control as a result of this activity but antagonistic effects on insecticide resistance should be considered when evaluating the potential of nanoparticles for cockroach control. © 2023 Society of Chemical Industry.