PULSE-LENGTH DEPENDENCE OF THE ELECTRICAL BREAKDOWN IN LIPID BILAYER-MEMBRANES
PULSE-LENGTH DEPENDENCE OF THE ELECTRICAL BREAKDOWN IN LIPID BILAYER-MEMBRANES
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DOI：
10.1016/0005-2736(80)90236-9
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
ZIMMERMANN, U
中科院分区：
文献类型：
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作者：
BENZ, R;ZIMMERMANN, U
在充电时间为10 ns~10μ的S人工脂双层膜上进行了电荷脉冲实验，如果膜的充电电压为100 mV，则膜电压与注入的电荷成线性函数关系。然而，如果膜被充电到1V的电压范围内，这种关系不再成立，并且出现可逆的高电导状态。这种状态被定义为电击穿，它不允许膜充电到高于击穿电压的电压Vc。在25°C下充电300 ns和5μS之间以及在40℃下充电100 ns和2μS之间，Vc强烈依赖于膜的充电时间，从1.2V(25°C)下降到0.5V(25°C)和从1 V(40°C)下降到0.4V(40℃)。对于这些范围以下和以上的其他充电时间，击穿电压似乎是恒定的。结果表明，击穿现象发生在10 ns以内。击穿电压的脉冲长度与电击穿机理的机电模型解释是一致的。然而，在膜的充电时间低于300 ns(25°C)和100 ns(40°C)的情况下，其他过程(例如本征能量)似乎是可能的。
Charge-pulse experiments were performed on artificial lipid bilayer membranes with charging times in the range between 10 ns and 10 μs. If the membranes are charged to voltages in the order of 100 mV, the membrane voltage at the end of the charge pulse is a linear function of the injected charge. However, if the membranes are charged to voltages in the range of 1 V, this relationship no longer holds and a reversible high conductance state occurs. This state is defined as an electrical breakdown and it does not allow the membranes to charge to higher voltages than the breakdown voltage, V c. Between charging times of 300 ns and 5 μs at 25° C and between 100 ns and 2 μs at 40° C, V c showed a strong dependence on the charging time of the membrane and decreased from 1.2 to 0.5 V (25° C) and from 1 to 0.4 V (40° C). For other charging times below and above these ranges, the breakdown voltage seemed to be constant. The results indicate that the breakdown phenomenon occurs in less than 10 ns. The pulse-length dependence of the breakdown voltage is consistent with the interpretation of the electrical breakdown mechanism in terms of the electromechanical model. However, it seems possible that below a charging time of the membrane of 300 ns (25° C) and 100 ns (40° C) other processes (such as the Born energy) become possible.