A Comprehensive Study of Palate Development in Miniature Pig
A Comprehensive Study of Palate Development in Miniature Pig
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DOI：
10.1002/ar.23597
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发表时间：
2017-08
期刊：
影响因子：
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通讯作者：
Lindong Sun;Jiangyi Wang;Huina Liu;Z. Fan;Songlin Wang;Juan Du
中科院分区：
文献类型：
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作者：
Lindong Sun;Jiangyi Wang;Huina Liu;Z. Fan;Songlin Wang;Juan Du
腭发育是面部发育中一个重要的形态发生事件，包括额鼻突和上颌骨的外侧和内侧鼻部分的融合。任何这些事件的脱轨可能导致腭裂，最常见的先天性颅面畸形。最近的研究表明，微型猪口腔颌面区域的显微解剖结构与人类非常相似，使用微型猪作为牙科和口腔面部研究的大型动物模型正在增加。然而，关于微型猪上颚发育的信息很少。在这里，我们用组织学和超微结构的方法描述了小型猪上颚的发育阶段。对E26、E30、E35、E40、E45和E50胚胎切片进行苏木精-伊红染色，并对所选标本进行电镜处理。小型猪上颚发育可分为4个阶段：30岁左右两侧腭架沿舌发育；舌舌上方E35处水平位置的标高；从E35-50开始在中线处建立双边大陆架接触；在E50处进行最后的融合步骤，类似于小鼠和人类。在超微结构水平上同步验证了微型猪腭不同发育阶段的组织学特征。本研究为小型猪腭裂发育机制的研究提供了第一手资料，为进一步研究该模型奠定了基础。中国生物医学工程学报，2017,30(3):1409 - 1419。©2017 Wiley期刊公司
Palate development is an important morphogenetic event in facial development, including the fusion of the lateral and medial nasal portions of the frontonasal process and maxilla. Derailments of any of these events may result in cleft palate, the most frequent congenital craniofacial abnormality. Recent research has shown that the microanatomy of the miniature pig oral maxillofacial region is quite similar to that of humans, and the use of miniature pigs as a large animal model for dental and orofacial research is increasing. Little information is available, however, about the development of the miniature pig palate. Here, using histological and ultrastructural methods, we describe the developmental stages of the palate in miniature pigs. Sections from E26, E30, E35, E40, E45, and E50 embryos were stained with hematoxylin–eosin, and selected specimens were also processed for electron microscopy. The development of the miniature pig palate can be divided into four stages: growth of the bilateral palatal shelves alongside the tongue at E30; elevation of the horizontal position above the tongue at E35; establishment of bilateral shelf contact at the midline from E35–50; and a final fusion step at E50, similar to the mouse and human. The histological characteristics of the miniature pig palate at different developmental stages were synchronously verified at the ultrastructural level. Our study provides a piece of first‐hand data regarding palate morphological organogenesis in the miniature pig and a foundation for further research with this model to explore mechanisms of cleft palate development. Anat Rec, 300:1409–1419, 2017. © 2017 Wiley Periodicals, Inc.