Light-driven water splitting for (bio-)hydrogen production:: photosystern 2 as the central part of a bioelectrochemical device
Light-driven water splitting for (bio-)hydrogen production:: photosystern 2 as the central part of a bioelectrochemical device
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DOI：
10.1562/2006-07-14-rc-969
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发表时间：
2006-09-01
影响因子：
3.3
通讯作者：
Roegner, Matthias
中科院分区：
文献类型：
--
作者：
Badura, Adrian;Esper, Berndt;Roegner, Matthias
为了建立一种利用光合作用水氧化产生(生物)氢的半人工装置，我们报道了将光系统2固定在电极表面的方法。为此，将从长蓝藻中遗传导入His标签的光系统2连接到末端含有Ni(II)-氮三乙酸基的硫醇酸盐修饰的金电极上。表面增强红外吸收光谱显示了光系统2的结合动力学，而表面等离子体共振测量使蛋白质的吸附数量得以定量。根据这些数据，计算出2的表面覆盖率为0.29pmolProtein Cm(-2)，这与电极表面形成单分子膜相一致。在光照下，观察到光电流的产生，电流密度高达14微安厘米(-2)。这种光电流明显依赖于光质量，表现出类似于孤立的光系统2的作用光谱。所获得的电流密度相当于在可比条件下在溶液中报告的最高析氧活性。
To establish a semiartificial device for (bio-)hydrogen production utilizing photosynthetic water oxidation, we report on the immobilization of a Photosystem 2 on electrode surfaces. For this purpose, an isolated Photosystem 2 with a genetically introduced His tag from the cyanobacterium Thermosynechococcus elongatus was attached onto gold electrodes modified with thiolates bearing terminal Ni(II)-nitrilotriacetic acid groups. Surface enhanced infrared absorption spectroscopy showed the binding kinetics of Photosystem 2, whereas surface plasmon resonance measurements allowed the amount of protein adsorbed to be quantified. On the basis of these data, the 2 surface coverage was calculated to be 0.29 pmol protein cm(-2), which is in agreement with the formation of a monomolecular film on the electrode surface. Upon illumination, the generation of a photocurrent was observed with current densities of up to 14 mu A cm(-2). This photocurrent is clearly dependent on light quality showing an action spectrum similar to an isolated Photosystem 2. The achieved current densities are equivalent to the highest reported oxygen evolution activities in solution under comparable conditions.