Investigation of expression and influence of CTGF and HO-1 in rats with diabetic retinopathy
Investigation of expression and influence of CTGF and HO-1 in rats with diabetic retinopathy
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DOI：
10.3892/etm.2019.8395
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发表时间：
2020-03-01
影响因子：
2.7
通讯作者：
Xue, Jinsong
中科院分区：
文献类型：
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作者：
Huang, Yongjian;Qian, Chaoxu;Xue, Jinsong
探讨CTGF和HO-1在糖尿病视网膜病变(DR)大鼠中的表达及其影响机制。选取雄性SD大鼠130只，随机分为对照组和DR组，每组65只。 DR组大鼠腹腔注射链脲佐菌素引起DR。建模成功55个，失败10个。成功的模型分别在第2、4、6个月处死。采用RT-qPCR技术检测各组大鼠视网膜CTGF、HO-1表达量,H&E染色观察大鼠视网膜灰度结构,TUNEL法检测视网膜细胞凋亡情况。 DR组第2个月时视网膜层紊乱，少数血管扩张。 DR组第4个月时视网膜内膜肿胀，神经节细胞排列不规则。 DR组第6个月血管扩张更明显，内膜水肿更严重，排列更不规则。 DR组大鼠视网膜细胞凋亡率在第2、4、6个月时逐渐升高，此后DR组视网膜中CTGF表达逐渐升高，而HO-1表达逐渐降低。但DR组第2、4、6个月大鼠CTGF、HO-1 mRNA表达量高于同期对照组。因此，CTGF和HO-1与大鼠DR的发生、发展相关，可作为DR治疗的靶点。
The expression and influence mechanism of CTGF and HO-1 in rats with diabetic retinopathy (DR) was investigated. One hundred and thirty male Sprague-Dawley (SD) rats were selected and randomly divided into the control group and DR group, with 65 rats in each group. DR was caused by intraperitoneal injection of streptozotocin in rats in the DR group. There were 55 successful models and 10 failed in the modelling. The successful models were sacrificed at the 2nd, 4th and 6th month, respectively. RT-qPCR technology was used for detection of the expression of CTGF and HO-1 in rat retina in each group, H&E staining for observation of the gradation structure in rat retina and TUNEL method for detection of apoptosis of retinal cells. In the DR group, the retina layers were disordered and a few blood vessels dilated at the 2nd month. In the DR group, the inner membrane of the retina swelled, and the ganglion cells were irregularly arranged at the 4th month. In the DR group, dilatation of the blood vessels was more obvious, the inner membrane edema was more severe, and the arrangement was more irregular at the 6th month. The retinal apoptosis rate of DR rats gradually increased at the 2nd, 4th and 6th month, after which, the CTGF expression gradually increased, but the HO-1 expression gradually decreased in retina in the DR group. However, the mRNA expression of CTGF and HO-1 in the rats at the 2nd, 4th and 6th month in the DR group was higher than that in the control group at the same period. Therefore, CTGF and HO-1 are associated with the occurrence and development of DR in rats and can be considered as targets for the treatment of DR.