Dissociated cell culture of cholinergic neurons from nucleus basalis of Meynert and other basal forebrain nuclei.
Dissociated cell culture of cholinergic neurons from nucleus basalis of Meynert and other basal forebrain nuclei.
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来自 Meynert 基底核和其他基底前脑核的胆碱能神经元的分离细胞培养物。
DOI：
10.1073/pnas.82.18.6325
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发表时间：
1985
影响因子：
11.1
通讯作者：
Yamaguchi,K
中科院分区：
文献类型：
--
作者：
Nakajima,Y;Nakajima,S;Obata,K;Carlson,CG;Yamaguchi,K
前脑基底核的胆碱能神经元变性被怀疑是阿尔茨海默病的原因。我们已经开发了来自这些核（Meynert 基底核、内侧间隔核和对角带核）的胆碱能神经元的分离培养物。用振动切片机制作前脑的脑切片，解剖、分离并培养前脑基底核。胆碱乙酰转移酶免疫细胞化学和乙酰胆碱酯酶细胞化学显示这些培养物中存在大胆碱能细胞（平均直径，20-25 微米）。大约 75% 的大神经元（直径 20 微米或更大）具有胆碱能。对这些大神经元进行了电生理学实验。神经元通常不会表现出自发放电，但稳定的去极化会产生一系列快速适应的动作电位。神经元对 L-谷氨酸的应用产生去极化反应。 P物质产生去极化（有时是超极化），并且在去极化过程中膜电阻增加。
Degeneration of cholinergic neurons from the basal forebrain nuclei is suspected to be the cause of Alzheimer disease. We have developed dissociated cultures of cholinergic neurons from these nuclei (the nucleus basalis of Meynert, the medial septal nucleus, and the diagonal band nuclei). Brain slices of the forebrains were made by a vibratome, and the basal forebrain nuclei were dissected out, dissociated, and cultured. Choline acetyltransferase immunocytochemistry and acetylcholinesterase cytochemistry revealed large cholinergic cells (average diameter, 20-25 micron) in these cultures. About 75% of large neurons (20 micron or larger in diameter) were cholinergic. Electrophysiological experiments were performed on these large neurons. The neurons usually did not show spontaneous firing, but steady depolarizations produced trains of action potentials, which adapted quickly. The neurons responded with depolarization to the application of L-glutamic acid. Substance P produced depolarization (sometimes hyperpolarization), and during the depolarization membrane resistance was increased.