Cells immobilized on patterns printed in DNA by an inkjet printer
Cells immobilized on patterns printed in DNA by an inkjet printer
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DOI：
10.1016/j.biomaterials.2011.01.066
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发表时间：
2011-05-01
期刊：
影响因子：
14
通讯作者：
Iwata, Hiroo
中科院分区：
文献类型：
--
作者：
Sakurai, Kengo;Teramura, Yuji;Iwata, Hiroo
将各种细胞自由地在基质上进行二维排列的能力将是分析细胞-细胞相互作用的有用工具。在这项研究中，我们旨在建立一种将细胞附着在衬底上的方法，其中的图案是由喷墨打印机绘制的。采用DNA共轭聚乙二醇-磷脂（DNA peg -脂）将多脱氧核糖核酸（DNA）固定在细胞表面，DNA peg -脂是peg -脂与单链DNA的两亲性共轭物。然后用喷墨打印机用互补DNA修饰基板表面。最后，通过DNA杂交将DNA固定的细胞附着在底物上。喷墨打印机的使用使我们能够以几百微米的分辨率在基板上准确地绘制DNA图案。DNA固定的细胞可以沿着底物上的DNA模式精确地附着。此外，利用不同的DNA序列可以同时附着多种细胞。我们的技术在干细胞研究中对细胞相互作用和分化诱导的分析有很大的前景。(C) 2011 Elsevier Ltd.版权所有。
The ability to two-dimensionally align various kinds of cells freely onto substrate would be a useful tool for analysis of cell-cell interactions. In this study, we aimed to establish a method for attaching cells to the substrate, in which the pattern is drawn by an inkjet printer. Poly-deoxyribonucleic acid (DNA) was immobilized onto the cell surface by use of DNA-conjugated poly(ethylene) glycol-phospholipid (DNA PEG-lipid), which is the amphiphilic conjugate of PEG-lipid and single-stranded DNA. The surface of the substrate was then modified with the complementary DNA using an inkjet printer. Finally, DNA-immobilized cells were attached onto the substrate through DNA hybridization. The use of the inkjet printer enabled us to draw the DNA pattern accurately on the substrate with a resolution of a few hundred micrometers. DNA-immobilized cells could be attached precisely along the DNA pattern on the substrate. In addition, various kinds of cells could be attached simultaneously by using various sequences of DNA. Our technique is promising for analysis of cell cell interactions and differentiation induction in stem cell research. (C) 2011 Elsevier Ltd. All rights reserved.