Expression of claudin-11,-23 in different gastric tissues and its relationship with the risk and prognosis of gastric cancer
Expression of claudin-11,-23 in different gastric tissues and its relationship with the risk and prognosis of gastric cancer
复制标题
Claudin-11、-23在不同胃组织中的表达及其与胃癌危险和预后的关系
DOI：
10.1371/journal.pone.0174476
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发表时间：
2017-03-28
期刊：
影响因子：
3.7
通讯作者：
Yuan, Yuan
中科院分区：
文献类型：
--
作者：
Lu, Youzhu;Jing, Jingjing;Yuan, Yuan
紧密连接蛋白在调节上皮细胞和内皮细胞的通透性和维持细胞极性方面起重要作用。本研究旨在探讨claudin-11、claudin-23在不同胃组织中的表达及其与胃癌临床病理参数和预后的关系。我们通过实时荧光定量PCR、免疫印迹和免疫组化比较了它们在配对癌组织和癌旁组织中的表达水平。结果表明，claudin-11、-23在胃癌癌旁组织中的表达明显高于癌组织。我们还通过免疫组织化学方法比较了它们在胃癌、浅表性胃炎和萎缩性胃炎组织中的表达水平。结果表明，claudin-11和claudin-23在浅表性胃炎中的表达明显高于萎缩性胃炎和胃癌。claudin-23在萎缩性胃炎中的表达明显低于胃癌，而claudin-11在萎缩性胃炎中的表达无明显差异。多因素Logistic回归分析显示，claudin-11与性别、吸烟、饮酒、H。pylori感染与Borrmann分级相关，claudin-23与血管癌栓相关。考克斯多因素生存分析显示，claudin-23阴性表达的胃癌患者总生存期明显延长。结论：claudin-11、claudin-23在胃癌中的表达明显下调。这些蛋白的异常表达与某些临床病理参数显著相关。特别是，claudin-23阳性表达与胃癌患者预后不良相关，因此可能作为患者生存的独立指标。
Claudins play an important role in regulating the permeability of epithelial and endothelial cells and in the maintenance of cell polarity. We aimed to investigate expression of claudin-11, -23 in different gastric tissues and its relationship with clinicopathologic parameters and prognosis of gastric cancer. We compared their expression levels in the paired cancerous tissues versus those in the adjacent noncancerous tissues by real-time PCR, western blotting and immunohistochemistry. The results showed that the expression of claudin-11, -23 was greatly increased in paracancerous gastric tissue compared with cancerous tissue. We also compared their expression levels of tissues from gastric cancer, superficial gastritis, and atrophic gastritis by immunohistochemistry. The results indicated that the expression of claudin-11 and 23 was significantly higher in superficial gastritis than that in atrophic gastritis and gastric cancer. The expression of claudin-23 was significantly lower in atrophic gastritis than that in gastric cancer, but no obviously difference was observed for claudin-11. As for analysis of clinicopathologic parameters of gastric cancer, logistic multiple regression indicated that claudin-11 was significantly associated with sex, smoking, alcohol, H. pylori infection and Borrmann classification while claudin-23 was significantly associated with vessel cancer embolus. Cox multivariate survival analysis indicated that gastric cancer patients with negative claudin-23 expression had significantly longer overall survival. In conclusion, the expression of claudin-11, -23 was remarkably downregulated in gastric cancer. Abnormal expression of these proteins was significantly correlated with some clinicopathologic parameters. In particular, claudin-23 positive expression was associated with poor prognostic outcomes of gastric cancer patients and may therefore serve as an independent prognosticator of patient survival.