Characterisation of bovine peroxisome proliferator-activated receptors gamma 1 and gamma 2: genetic mapping and differential expression of the two isoforms.
Characterisation of bovine peroxisome proliferator-activated receptors gamma 1 and gamma 2: genetic mapping and differential expression of the two isoforms.
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牛过氧化物酶体增殖物激活受体 γ 1 和 γ 2 的表征：两种亚型的遗传图谱和差异表达。
DOI：
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发表时间：
1997
影响因子：
3.1
通讯作者：
S. Lien
中科院分区：
文献类型：
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作者：
H. Sundvold;A. Brzozowska;S. Lien
In this report we describe the isolation and characterisation of the cDNAs encoding two isoforms of peroxisome proliferator-activated receptor gamma (PPAR gamma), gamma 1 and gamma 2, in cattle. The cDNA sequences show strong conservation with the corresponding sequences reported in other species. The distribution of PPAR gamma mRNAs in various bovine tissues was investigated using Northern blot analysis. The highest expression was detected in adipose tissue with about equal amounts of the both transcripts while a differential expression was found in other tissues investigated. PPAR gamma 1 was expressed at relatively high levels in bovine spleen and lung and to a lower extent in ovary, mammary gland, and small intestine. The amount of PPAR gamma 2 was apparently lower than that of PPAR gamma 1 in spleen, lung, and ovary. These results indicate a modulation of tissue distribution for the two transcripts in cattle. Using genetic linkage analysis we have also assigned the PPAR gamma gene to bovine chromosome 22.
