Y-box Binding Protein 1 Is Involved in Regulating the G2/M Phase of the Cell Cycle
Y-box Binding Protein 1 Is Involved in Regulating the G2/M Phase of the Cell Cycle
复制标题
DOI：
10.21873/anticanres.11490
复制
发表时间：
2017-04-01
影响因子：
2
通讯作者：
Naemura, Madoka
中科院分区：
文献类型：
--
作者：
Kotake, Yojiro;Arikawa, Nagisa;Naemura, Madoka
背景/目的：转录因子Y-box-binding protein 1（YB 1）在许多类型的人类癌症中过表达。YB 1通过控制G（1）调节因子的转录来调节细胞周期的G（1）期。在此，我们报道YB 1也参与调节G（2）/M期。材料和方法：对YB 1缺失的TKO细胞进行定量逆转录-聚合酶链反应和细胞周期分析。使用抗YB 1抗体进行RNA免疫沉淀（RIP）芯片测定。对经扩增的RNA进行微阵列分析。结果：沉默YB 1抑制了TKO细胞的增殖，使其失去了G（1）期阻滞所需的机制。细胞周期分析表明，沉默YB 1可使细胞周期阻滞于G（2）/M期。RIP-chip分析显示YB 1与多个细胞周期相关基因的mRNA相关，包括G（2）/M期调节因子。结论：YB 1通过调控多个细胞周期相关基因，对G1期和G2/M期细胞进行正调控。
Background/Aim: The transcription factor Y-box-binding protein 1 (YB1) is overexpressed in many types of human cancers. YB1 regulates the G(1) phase of the cell cycle by controlling transcription of G(1) regulators. Here, we report that YB1 is also involved in regulating G(2)/M phase. Materials and Methods: YB1-depleted TKO cells were subjected to quantitative reverse transcription-polymerase chain reaction and cell-cycle analysis. RNA immuno-precipitation (RIP)-chip assay was performed using anti-YB1 antibodies. Precipitated RNAs were subjected to microarray analysis. Results: Silencing YB1 inhibited the proliferation of TKO cells, which lost the machinery required for G(1) phase arrest. Cell-cycle analysis showed that silencing YB1 caused G(2)/M phase cellcycle arrest. RIP-chip assay showed that YB1 associated with mRNA of multiple cell-cycle-related genes, including G(2)/M phase regulators. Conclusion: YB1 positively regulates not only the G(1) phase but also G(2)/M phase by regulating multiple cell-cycle-related genes.