Specific volumes of lipids in fully hydrated bilayer dispersions.
Specific volumes of lipids in fully hydrated bilayer dispersions.
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DOI：
10.1016/0005-2736(88)90153-8
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发表时间：
1988-02
期刊：
影响因子：
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通讯作者：
M. Wiener;S. Tristram-Nagle;D.Alan Wilkinson;L. E. Campbell;J. F. Nagle
中科院分区：
文献类型：
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作者：
M. Wiener;S. Tristram-Nagle;D.Alan Wilkinson;L. E. Campbell;J. F. Nagle
严格研究了在多层分散体中获得脂质绝对比体积的中性浮力方法。对照实验表明，2H2O 与 H2O 没有优先分配到脂质体中，并且样品的一些热力学性质（例如主转变的焓变和体积变化）随着溶剂的氘化而变化很小。讨论了层间空间中溶剂的分子体积与本体溶液中的分子体积基本相同的假设；并且它被证明引入了相当小的修正。先前的程序已被修改，以避免低透水性相中可能的动力学限制。结论是，双层脂质分子体积的测量精度优于 0.002 nm3(2Å3)，小于典型脂质分子体积的 0.2%。
The neutral buoyancy method of obtaining absolute specific volumes of lipid in multilamellar dispersions is critically investigated. Control experiments show that there is no preferential partitioning of2H2O vs. H2O into the liposomes, and several thermodynamic properties of the samples, such as the enthalpy change and the volume change of the main transition, are changed very little with deuteration of the solvent. The assumption that the molecular volume of the solvent in the interlamellar space is essentially the same as in bulk solution is discussed; and it is shown to introduce rather small corrections. Previous procedures have been modified to avoid possible kinetic limitations in phases with low water permeability. It is concluded that the molecular volume of lipid in bilayers can be obtained to an accuracy better than 0.002 nm3(2Å3) which is less than 0.2% of typical molecular volumes of lipids.