Adaptation of Bacillus subtilis cells to Archean-like UV climate: relevant hints of microbial evolution to remarkably increased radiation resistance.
Adaptation of Bacillus subtilis cells to Archean-like UV climate: relevant hints of microbial evolution to remarkably increased radiation resistance.
复制标题
枯草芽孢杆菌细胞对类太古宙紫外线气候的适应：微生物进化与显着增强的耐辐射性的相关提示。
DOI：
10.1089/ast.2009.0455
复制
发表时间：
2010
期刊：
影响因子：
4.2
通讯作者：
P. Rettberg
中科院分区：
文献类型：
--
作者：
M. Wassmann;R. Moeller;G. Reitz;P. Rettberg
在空间实验ADAPT的初步研究中，（“微生物对不同空间和行星紫外线气候条件的分子适应战略”），枯草芽孢杆菌168细胞在周期性多色紫外线照射下连续培养700代（200-400 nm）模拟早期地球上建议的紫外线辐射环境，在太古代期间，当地球缺乏重要的臭氧层。在紫外线胁迫下进化的种群比祖先和非紫外线进化的种群具有约3倍的抗性。紫外线进化的细胞对电离辐射的抵抗力是其未暴露于紫外线的进化亲属和祖先的7倍。除了获得增加的紫外线抗性，进一步的变化，在微生物应激反应过氧化氢，盐度增加，和干燥的紫外线进化的细胞中观察到。这表明，紫外线敏感的祖先细胞能够适应周期性施加的紫外线胁迫，通过进化的细胞具有增加的紫外线抗性水平和进一步增强对其他环境压力的反应，从而使它们能够在极端紫外线辐射下生存和繁殖作为选择压力。
In a precursory study for the space experiment ADAPT ("Molecular adaptation strategies of microorganisms to different space and planetary UV climate conditions"), cells of Bacillus subtilis 168 were continuously cultured for 700 generations under periodic polychromatic UV irradiation (200-400 nm) to model the suggested UV radiation environment on early Earth at the origin of the first microbial ecosystem during the Archean eon when Earth lacked a significant ozone layer. Populations that evolved under UV stress were about 3-fold more resistant than the ancestral and non-UV-evolved populations. UV-evolved cells were 7-fold more resistant to ionizing radiation than their non-UV-exposed evolved relatives and ancestor. In addition to the acquired increased UV resistance, further changes in microbial stress response to hydrogen peroxide, increased salinity, and desiccation were observed in UV-evolved cells. This indicates that UV-sensitive ancestral cells are capable of adapting to periodically applied UV stress via the evolution of cells with an increased UV resistance level and further enhanced responses to other environmental stressors, which thereby allows them to survive and reproduce under extreme UV radiation as a selection pressure.
简单非结构化环境中的微生物进化：大肠杆菌的遗传分化。
影响因子：
3.3
作者：
Rosenzweig,RF;Sharp,RR;Treves,DS;Adams,J
通讯作者：
Adams,J