Decreased circulating levels of active ghrelin are associated with increased oxidative stress in obese subjects
Decreased circulating levels of active ghrelin are associated with increased oxidative stress in obese subjects
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DOI：
10.1530/eje.1.01977
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发表时间：
2005-09-01
影响因子：
5.8
通讯作者：
Adachi, Y
中科院分区：
文献类型：
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作者：
Suematsu, M;Katsuki, A;Adachi, Y
目的：为了探讨活动ghrelin和氧化stressinobesis.Design之间的关系：我们测量了血浆水平的游离8-epi-PGF-2 α（8-epi-PGF（2 α），一个可靠的和系统的氧化应激标志物）和活动形式的ghrelin在17个肥胖和17个正常人。生长素释放肽的生物活性形式使用市售放射免疫测定试剂盒测量，游离8-epi-PGF（2 α）使用酶免疫测定试剂盒测量。循环中活性ghrelin水平显著降低（20.4 ± 2.6 vs 40.9 ± 3.9 fmol/ml，P < 0.01），而8-epi-PGF（2 α）显著升高（61.5 ± 9.6 vs 17.3 ± 3.4 pg/ml，P < 0.01）。血浆活性ghrelin和8-epi-PGF（2 α）水平在肥胖组（r =-0.507，P < 0.05）和所有组（r = 0.577，P < 0.01）均显著相关。多因素分析显示，所有受试者血浆中活性ghrelin和8-epi-PGF（2 α）水平均呈显著独立相关（F = 7.888，P < 0.01）。低循环水平的活性生长激素释放肽可能会增强肥胖受试者的氧化应激和动脉粥样硬化过程。
Objective: To investigate the relationship between active ghrelin and oxidative stress in obese subjects.Design: We measured the plasma levels of free 8-epi-prostaglandin F-2 alpha (8-epi-PGF(2 alpha), a reliable and systemic marker of oxidative stress) and the active form of ghrelin in 17 obese and 17 normal subjects. The biologically active forms of ghrelin were measured-using a commercially available radio-immunoassay kit and free 8-epi-PGF(2 alpha) was measured using an enzyme immunoassay kit.Results: The circulating level of active ghrelin was significantly decreased (20.4+/-2.6 vs 40.9 +/- 3.9 fmol/ml, P < 0.01) while that of 8-epi-PGF(2 alpha) was significantly increased (61.5 +/- 9.6 vs 17.3 +/- 3.4 pg/ml, P < 0.01) in obese subjects compared with normal subjects. The plasma levels of active ghrelin and 8-epi-PGF(2 alpha) were significantly correlated in obese (r = -0.507, P < 0.05) and in all (r = 0.577, P < 0.01) subjects. Multivariate analysis showed that the plasma levels of active ghrelin and 8-epi-PGF(2 alpha) were significantly and independently correlated in all subjects (F = 7.888, P < 0.01).Conclusions: There is an inverse correlation between circulating levels of active ghrelin and oxidative stress in obesity. Low circulating levels of active ghrelin may enhance oxidative stress and the process of atherosclerosis in obese subjects.