Bacteria in the intestinal tract of different species of arthropods
Bacteria in the intestinal tract of different species of arthropods
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DOI：
10.1007/s002489900021
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发表时间：
1997-05-01
影响因子：
3.6
通讯作者：
vanderDrift, C
中科院分区：
文献类型：
--
作者：
Cazemier, AE;Hackstein, JHP;vanderDrift, C
测定了属于7个不同目的12种节肢动物肠道内的细菌数量，以获得有关肠道细菌对食物消化的重要性的信息。因此，开发了一种简单有效的直接计数节肢动物胃肠道中 4', 6-二脒基-2-苯基吲哚 (DAPI) 染色细菌的方法。在四焦磷酸钠（PPi）存在的情况下，通过超声波处理可以将肠道细菌从肠壁释放出来。前肠细菌计数范围为 0.2 至 3.6 x 10(9) (mi gut)(-1)，中肠细菌计数范围为 0.2 至 28 x 10(9) (ml gut)(-1)，后肠细菌计数范围为 0.1 至 190 x 10(9) (ml gut)(-1)。 Hylotrupes bajules 幼虫的前肠和后肠没有细菌； Eurycanta calcarata 和 Schistocerca gragaria 的整个肠道仅含有少量细菌。几种节肢动物肠道某些部分的细菌数量足够高，表明它们对消化过程有潜在的贡献。
The number of bacteria in the intestine of 12 species of arthropods, belonging to 7 different orders, was determined to obtain information about the significance of intestinal bacteria for the digestion of food. Therefore, a simple and effective method for direct counts of 4', 6-diamidino-2-phenylindole (DAPI) stained bacteria from the gastrointestinal tract of arthropods was developed. The intestinal bacteria could be released from the gut wall by ultrasonic treatment in the presence of sodium tetrapyrophosphate (PPi). The bacterial counts ranged between 0.2 and 3.6 x 10(9) (mi gut)(-1) in the foregut, 0.2 and 28 x 10(9) (ml gut)(-1) in the midgut, and 0.1 and 190 x 10(9) (ml gut)(-1) in the hindgut. The foregut and hindgut of Hylotrupes bajules larvae were devoid of bacteria; the whole intestinal tract of Eurycanta calcarata and Schistocerca gragaria contained only low numbers of bacteria. The population of bacteria in some parts of the intestinal tract of several arthropods were high enough to suggest a potential contribution to digestive processes.