Crystallization and preliminary X-ray crystallographic studies of HLA-A*1101 complexed with an HIV-1 decapeptide.
Crystallization and preliminary X-ray crystallographic studies of HLA-A*1101 complexed with an HIV-1 decapeptide.
复制标题
HLA-A*1101 与 HIV-1 十肽复合物的结晶和初步 X 射线晶体学研究。
DOI：
10.1107/s0907444902007928
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Bouvier,Marlene
中科院分区：
文献类型：
--
作者：
Li,Lenong;Promadej,Nattawan;McNicholl,JanetM;Bouvier,Marlene
A major goal of vaccine research for the prevention of AIDS is to determine the immune correlates of protection against HIV-1 infection. In this context, it is of interest to understand how HLA-A*1101, a significantly more prevalent class I allele in a cohort of highly HIV-1-exposed persistently seronegative individuals, functions in relation to protective immunity to HIV-1. Towards this goal, a soluble recombinant HLA-A*1101 molecule has been expressed and used to assemble a complex with β2-microglobulin and a Nef decapeptide. The HLA-A*1101/β2m/Nef complex was crystallized by the hanging-drop vapor-diffusion method. The crystal formed in the monoclinic space group P21, with unit-cell parameters a = 77.2, b = 88.5, c = 64.8 Å, β = 90.1°, and contains two molecules in the asymmetric unit. A data set to 2.2 Å resolution was collected and structure determination by molecular replacement is currently in progress. Understanding the three-dimensional structure of the HLA-A*1101/β2m/Nef complex may provide insight into the functional role of this class I allele in relation to protective immunity to HIV-1.
影响因子：
1.5
作者：
B. Sriwanthana;T. Hodge;T. Mastro;C. Dezzutti;Kyle B. Bond;H. Stephens;L. Kostrikis;K. Limpakarnjanarat;N. Young;S. Qari;R. Lal;Dasnayanee Chandanayingyong;M. JANETM.;Nicholl
通讯作者：
B. Sriwanthana;T. Hodge;T. Mastro;C. Dezzutti;Kyle B. Bond;H. Stephens;L. Kostrikis;K. Limpakarnjanarat;N. Young;S. Qari;R. Lal;Dasnayanee Chandanayingyong;M. JANETM.;Nicholl
影响因子：
1.5
作者：
Bond, KB;Sriwanthana, B;McNicholl, JM
通讯作者：
McNicholl, JM