Elastic modulus and hardness of cortical and trabecular bone lamellae measured by nanoindentation in the human femur
Elastic modulus and hardness of cortical and trabecular bone lamellae measured by nanoindentation in the human femur
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DOI：
10.1016/s0021-9290(99)00111-6
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发表时间：
1999-10-01
影响因子：
2.4
通讯作者：
Goldstein, SA
中科院分区：
文献类型：
--
作者：
Zysset, PK;Guo, XE;Goldstein, SA
骨组织的力学性能是由组成以及结构、微观结构和纳米结构组织决定的。本研究的目的是量化骨板层水平的弹性特性，并比较来自人股骨骨干和股骨颈的骨、间质和小梁微观结构的这些特性。采用纳米压痕技术和定制的灌溉系统，同时测量了将金刚石尖端压入湿润骨组织500纳米处的力和位移。根据卸载曲线计算各向同性弹性模量，假设泊松比为0.3，硬度定义为最大力除以相应的接触面积。弹性模量范围从74岁女性股骨颈小梁组织的6.9 +/- 4.3 GPa到69岁女性骨干皮质间质组织的25.0 +/- 4.3 GPa。平均弹性模量受片层类型（p < 10(-6)）和供体（p < 10(-6)）的显著影响。片层类型与供体之间的相互作用也非常显著（p < 10(-6)）。硬度与弹性模量在不同片层和供体类型间的分布相似，但统计对比较低。结论是，骨组织的纳米结构在不同的板层类型、解剖部位和个体之间必然存在很大差异，并表明组织异质性在骨的脆弱性和适应性中具有潜在的重要性。1999爱思唯尔科学有限公司版权所有。
The mechanical properties of bone tissue are determined by composition as well as structural, microstructural and nanostructural organization. The aim of this study was to quantify the elastic properties of bone at the lamellar level and compare these properties among osteonal, interstitial and trabecular microstructures from the diaphysis and the neck of the human femur. A nanoindentation technique with a custom irrigation system was used for simultaneously measuring force and displacement of a diamond tip pressed 500 nm into the moist bone tissue. An isotropic elastic modulus was calculated from the unloading curve with an assumed Poisson ratio of 0.3, while hardness was defined as the maximal force divided by the corresponding contact area. The elastic moduli ranged from 6.9 +/- 4.3 GPa in trabecular tissue from the femoral neck of a 74 yr old female up to 25.0 +/- 4.3 GPa in interstitial tissue from the diaphyseal cortex of a 69 yr old female. The mean elastic modulus was found to be significantly influenced by the type of lamella (p < 10(-6)) and by donor (p < 10(-6)). The interaction between the type of lamella and the donor was also highly significant(p < 10(-6)). Hardness followed a similar distribution as elastic modulus among types of lamellae and donor, but with lower statistical contrast. It is concluded that the nanostructure of bone tissue must differ substantially among lamellar types, anatomical sites and individuals and suggests that tissue heterogeneity is of potential importance in bone fragility and adaptation. (C) 1999 Elsevier Science Ltd. All rights reserved.