A bifunctional AuNP probe-based enzyme-linked immunosorbent assay for facile and ultrasensitive detection of trace zearalenone in coix seed
A bifunctional AuNP probe-based enzyme-linked immunosorbent assay for facile and ultrasensitive detection of trace zearalenone in coix seed
复制标题
基于双功能 AuNP 探针的酶联免疫吸附测定法，可轻松、超灵敏地检测薏苡仁中的痕量玉米赤霉烯酮
DOI：
10.1016/j.microc.2022.108152
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发表时间：
2022-11-16
影响因子：
4.8
通讯作者：
Zhang, Lei
中科院分区：
文献类型：
--
作者：
Huang, Rentang;Huang, Yuxin;Zhang, Lei
Herein, we present a bifunctional gold nanoparticles (AuNP) probe-based enzyme-linked immunosorbent assay (nano-ELISA) for detecting zearalenone (ZEN) in the popular edible and medicinal food coix seed. This nano-ELISA takes advantage of the convenient labeling property of AuNPs, which allows the horseradish peroxidase and antibody were successively immobilized onto AuNPs by electrostatic adsorption to construct a bifunctional probe for identification and signal amplification. In our model system, a time-saving direct competitive ELISA scheme was selected, in which mutual optimization of biomolecules, a two-stage process for incubation, and comparison of different particle sizes were performed to reach higher sensitivity. The proposed method achieves a limit of detection of 8 mu g/kg and a limit of quantitation of 22 mu g/kg, which is an approximately 7-fold and 5 -fold improvement over the traditional ELISA, respectively. The calibration curve for the analyte was linear over the concentration range of 22-508 mu g/kg. The recoveries for ZEN spiked coix seed samples ranged from 98.67 to 113.5 % with a relative standard deviation of less than 8 %. Moreover, the developed nano-ELISA could over-come the interference from the sample matrix. A good correlation was observed between the results from nano-ELISA and liquid chromatography-tandem mass spectrometry (r2 = 0.9757, n = 69), which paves the useful and practicable way for accurate bioanalysis for trace hazardous substances in food.
