CLONING OF AN ARABIDOPSIS-THALIANA CDNA CODING FOR FARNESYL DIPHOSPHATE SYNTHASE BY FUNCTIONAL COMPLEMENTATION IN YEAST
CLONING OF AN ARABIDOPSIS-THALIANA CDNA CODING FOR FARNESYL DIPHOSPHATE SYNTHASE BY FUNCTIONAL COMPLEMENTATION IN YEAST
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DOI：
10.1007/bf00019499
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发表时间：
1994-12-01
影响因子：
5.1
通讯作者：
KARST, F
中科院分区：
文献类型：
--
作者：
DELOURME, D;LACROUTE, F;KARST, F
从拟南芥cDNA文库中克隆了一段编码法尼酯二磷酸合成酶的cDNA，该合成酶是由二磷酸异戊烯基和二磷酸二甲基烯基合成C15类异戊二烯二磷酸的酶。拟南芥cDNA也能够补充erg20缺失酵母突变体的致死表型。从全长1.22 kb的cDNA核苷酸序列推断，该多肽含有343个氨基酸，相对分子质量为39689。预测的氨基酸序列与酵母、大鼠和人FPP合成酶的一致性约为50%。Southern blot分析表明，拟南芥可能含有一个法尼酯二磷酸合成酶基因。
A cDNA encoding farnesyl diphosphate synthase, an enzyme that synthesizes C15 isoprenoid diphosphate from isopentenyl diphosphate and dimethylallyl diphosphate, was cloned from an Arabidopsis thaliana cDNA library by complementation of a mutant of Saccharomyces cerevisiae deficient in this enzyme. The A. thaliana cDNA was also able to complement the lethal phenotype of the erg20 deletion yeast mutant. As deduced from the full-length 1.22 kb cDNA nucleotide sequence, the polypeptide contains 343 amino acids and has a relative molecular mass of 39 689. The predicted amino acid sequence presents about 50% identity with the yeast, rat and human FPP synthases. Southern blot analyses, indicate that A. thaliana probably contains a single gene for farnesyl diphosphate synthase.