Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert)
Light-induced biochemical variations in secondary metabolite production and antioxidant activity in callus cultures of Stevia rebaudiana (Bert)
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DOI：
10.1016/j.jphotobiol.2015.11.015
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发表时间：
2016-01-01
影响因子：
5.4
通讯作者：
Ahmad, Nisar
中科院分区：
文献类型：
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作者：
Ahmad, Naveed;Rab, Abdur;Ahmad, Nisar
甜菊糖（S. rebaudiana）是一种非常重要的药用和商业用途的植物。光是引起形态发生电位和生化反应波动的主要刺激物之一。在本研究中，我们研究了不同光谱光对金盏花愈伤组织生物量积累和次生代谢物产生的影响。将叶片外植体置于Murashige和Skoog （MS）培养基上，并暴露在各种光谱光下。6-苄基腺嘌呤（BA）和2,4 -二氯苯氧基乙酸（2,4 -d; 2.0 mg l(-1)）诱导愈伤组织。对照光（16/8 h）的胼胝质形成反应最佳（92.73%）。与其他色光相比，对照培养物在生长动力学第18天的延长对数期中表现出最大的生物量积累（5.78 g l(-1)）。蓝光培养提高了总酚含量（TPC, 102.32 μ g/g DW）、总黄酮含量（TFC, 22.07 μ g/g DW）和总抗氧化能力（TAC, 11.63 μ g/g DW）。相反，绿灯和红灯提高了还原能力测定(RPA; 0.71 Fe（II) g(-1) DW）和dph自由基清除活性（DRSA; 80%）。因此，我们得出结论，利用彩色光是一种很有前途的策略，可以提高黄芪愈伤组织中抗氧化次生代谢物的产生。(C) 2015 Elsevier B.V.版权所有
Stevia rebaudiana (S. rebaudiana) is a very important species with worldwide medicinal and commercial uses. Light is one of the major elicitors that fluctuate morphogenic potential and biochemical responses. In the present study, we investigated the effect of various spectral lights on biomass accumulation and secondary metabolite production in callus cultures of S. rebaudiana. Leaf explants were placed on Murashige and Skoog (MS) medium and exposed to various spectral lights. 6-Benzyle adenine (BA) and 2, 4-dichlorophenoxy acetic acid (2, 4-D; 2.0 mg l(-1)) were used for callus induction. The control light (16/8 h) produced optimum callogenic response (92.73%) than other colored lights. Compared to other colored lights, control grown cultures displayed maximum biomass accumulation (5.78 g l(-1)) during a prolonged log phase at the 18th day of growth kinetics. Cultures grown under blue light enhanced total phenolic content (TPC; 102.32 mu g/g DW), total flavonoid content (TFC; 22.07 mu g/g DW) and total antioxidant capacity (TAC; 11.63 mu g/g DW). On the contrary, green and red lights improved reducing power assay (RPA; 0.71 Fe(II) g(-1) DW) and DPPH-radical scavenging activity (DRSA; 80%). Herein, we concluded that the utilization of colored lights is a promising strategy for enhanced production of antioxidant secondary metabolites in callus cultures of S. rebaudiana. (C) 2015 Elsevier B.V. All rights reserved.