Facile preparation of magnetic carbon nanotubes-immobilized lipase for highly efficient synthesis of 1,3-dioleoyl-2-palmitoylglycerol-rich human milk fat substitutes
Facile preparation of magnetic carbon nanotubes-immobilized lipase for highly efficient synthesis of 1,3-dioleoyl-2-palmitoylglycerol-rich human milk fat substitutes
复制标题
轻松制备磁性碳纳米管固定化脂肪酶，用于高效合成富含 1,3-二油酰-2-棕榈酰甘油的母乳脂肪替代品
DOI：
10.1016/j.foodchem.2017.01.129
复制
发表时间：
2017
期刊：
影响因子：
8.8
通讯作者：
Xiao Junyong
中科院分区：
文献类型：
--
作者：
Zheng Mingming;Wang Shi;Xiang Xia;Shi Jie;Huang Juan;Deng Qianchun;Huang Fenghong;Xiao Junyong
在这项研究中，念珠菌脂解脂肪酶（CLL）通过疏水性和阳离子交换相互作用固定在磁性多壁碳纳米管（mMWCNT）上。所得的固定化 CLL 比游离形式表现出更好的热稳定性、生物催化剂活性并且更容易回收。开发了一种使用固定化 CLA 作为生物催化剂，用油酸 (OA) 高效酶促酸解三棕榈酸 (PPP) 来生产富含 OPO 的 TAG 的方法。在优化条件下（2%水、20 mg/ml酶、1:6 PPP/OA、50°C、2 h），最终产品中OPO含量达到46.5%。CLL@mMWCNTs比商业脂肪酶具有更好的活性和操作稳定性。更重要的是，CLL@mMWCNTs的可行性也在以猪油和重组棕榈油为原料的富含OPO的TAG的实际生产中得到了验证。这些结果表明，CLL@mMWCNTs 是一种很有前景的生物催化剂，可用于生产富含 OPO 的 TAG，并将有助于婴儿配方奶粉行业。
In this study,Candida lipolyticalipase (CLL) was immobilized on magnetic multi-walled carbon nanotubes (mMWCNTs) via hydrophobic and cation-exchange interaction. The resultant immobilizedCLLshowed much better thermal stability, biocatalyst activity and easier recycling than the free form. A method for efficient enzymatic acidolysis of tripalmitin (PPP) with oleic acid (OA), to produce OPO-rich TAGs, was developed, using the immobilizedCLLas the biocatalyst. Under optimized conditions (2% water, 20 mg/ml enzyme, 1:6 PPP/OA, 50 °C, 2 h), the content of OPO in the final product reached 46.5%.CLL@mMWCNTs had a better activity and manipulative stability than commercial lipases. More importantly, the feasibility ofCLL@mMWCNTs was also validated in the practical production of OPO-rich TAGs, using lard and restructured palm oil as the raw material. These results suggest thatCLL@mMWCNTs is a promising biocatalyst for the OPO-rich TAGs production and will be helpful for the infant formula industry.