Immunostimulatory AIE Dots for Live-Cell Imaging and Drug Delivery
Immunostimulatory AIE Dots for Live-Cell Imaging and Drug Delivery
复制标题
用于活细胞成像和药物输送的免疫刺激 AIE 点
DOI：
10.1021/acsami.1c02128
复制
发表时间：
2021
影响因子：
9.5
通讯作者：
Shen Jianlei
中科院分区：
文献类型：
--
作者：
Gu Peilin;Chen Bin;Zhai Tingting;Li Qian;Zuo Xiaolei;Wang Lihua;Qin Anjun;Zhou Yi;Shen Jianlei
纳米药物载体的机械性能在调节纳米生物相互作用中起着关键作用。例如，较软的纳米颗粒的上级可变形性使它们能够有效地通过生物过滤器，促进它们的长血液循环和更好的肿瘤渗透。然而，作为一种新型的纳米载体系统，软纳米颗粒从细胞中的消除效率的研究很少。在这里，我们报告了一个简单的策略，通过自组装的两亲性聚集诱导发射（AIE）荧光团制备软发光纳米粒子。所制备的软质AIE点具有较强的光发射（量子产率为27.1%），并且可以真实的实时地揭示纳米颗粒的包封和排泄过程。细胞结果表明，软纳米粒子可以大大提高纳米材料进入细胞的转移速度，并通过牺牲软AIE点加速它们从细胞中消除。我们还表明，软AIE点加载胞嘧啶磷酸鸟嘌呤（CpG）寡脱氧核苷酸可以诱导强烈的免疫刺激作用，产生高水平的各种促炎细胞因子，包括肿瘤坏死因子（TNF）-R，白细胞介素（IL）-6，IL-12。这项工作展示了一种新的设计策略，用于合成一种软纳米载体系统，该系统可以有效地将药物递送到细胞中，然后迅速从细胞中消除。
The mechanical properties of nanoscale drug carriers play critical roles in regulating nano–bio interactions. For example, the superior deformability of the softer nanoparticles enables them to pass through the biofilters efficiently, facilitating their long blood circulation and better tumor penetration. However, as a novel nanocarrier system, the elimination efficiency of soft nanoparticles from cells is poorly investigated. Here, we report a facile strategy to prepare soft luminescent nanoparticles through self-assembly of amphiphilic aggregation-induced emission (AIE) fluorophores. The prepared soft AIE dots exhibit strong light emission (quantum yield, ∼27.1%) and can reveal the encapsulation and excretion process of NPs in real time. The cell results showed that soft NPs can greatly increase the transfer speed of nanomaterials into cells and accelerate their elimination from cells through the sacrifice of soft AIE dots. We also show that soft AIE dots loaded with cytosine–phosphate–guanine (CpG) oligodeoxynucleotides can induce strong immunostimulatory effects, producing a high level of various proinflammatory cytokines including tumor necrosis factor (TNF)-R, interleukin (IL)-6, and IL-12. This work demonstrates a new design strategy for synthesizing a soft nanocarrier system that can deliver drugs into cells efficiently and then be eliminated from cells quickly.