Expression of phospholipase D2 in human colorectal carcinoma.
Expression of phospholipase D2 in human colorectal carcinoma.
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DOI：
10.3892/or.18.5.1329
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发表时间：
2007-11
期刊：
影响因子：
4.2
通讯作者：
Masaru Saito;M. Iwadate;M. Higashimoto;K. Ono;Y. Takebayashi;S. Takenoshita
中科院分区：
文献类型：
--
作者：
Masaru Saito;M. Iwadate;M. Higashimoto;K. Ono;Y. Takebayashi;S. Takenoshita
磷脂酶D(PLD)催化磷脂酰胆碱(PC)水解生成磷脂酸(PA)和胆碱。PA是细胞增殖的第二信使，因此PLD被认为在肿瘤发生中起重要作用。据报道，与邻近的非肿瘤组织相比，PLD在人类乳腺癌、胃癌、肾细胞癌和结直肠癌中的活性升高。PLD活性还与乳腺癌的核分级、胃癌的肿瘤大小、结直肠癌的淋巴结转移和更深的侵袭有关。然而，每项研究中的病例数量都很少。本研究旨在探讨PLD2在结直肠癌中的表达水平及其与临床病理特征的关系。手术切除结直肠癌97例。实时定量聚合酶链式反应检测PLD2基因的表达水平。通过对2000-2004年期间的死亡率随访数据进行生存分析，分析了PLD2表达水平与结直肠癌患者预后的相关性。PLD在不同肿瘤中的表达水平不同。PLD表达水平与肿瘤大小显著相关(P<；0.05)，与淋巴结转移、侵袭程度、病理分型、远处转移及Dukes‘s分期无关。PLD的表达水平也与结直肠癌患者的生存期显著相关(P<；0.05)。提示PLD2在结直肠癌的发生发展中起重要作用，可作为结直肠癌治疗的靶点。
Phospholipase D (PLD) catalyzes the hydrolysis of phosphatidylcholine (PC) to generate phosphatidic acid (PA) and choline. PA acts as a second messenger in cell proliferation; therefore PLD is believed to play an important role in carcinogenesis. PLD activity has been reported to be elevated in human breast, gastric, renal cell and colorectal carcinomas, compared with adjacent non-neoplastic tissues. The activity of PLD was also correlated with nuclear grade in breast cancer, tumor size in gastric carcinoma, and nodal involvement and deeper invasion in colorectal carcinoma. However, the number of cases in each study was small. The aim of this study was to investigate the expression level of PLD2 and its association with clinicopathological features in human colorectal carcinoma. Ninety-seven colorectal carcinomas were obtained from surgery. Expression level of PLD2 was assessed by real-time PCR. The prognostic relevance of PLD2 expression level in patients with colorectal carcinoma was also analyzed by the survival analysis of mortality follow-up data covering the period 2000-2004. PLD expression level was varied from tumor to tumor. Expression level of PLD was significantly correlated with tumor size (P<0.05); it was independent of lymph node metastasis, extent of invasion, pathological classification, distant metastasis and Dukes' stage. PLD expression level was also significantly correlated with survival of patients with colorectal carcinoma (P<0.05). These findings suggested that PLD2 plays an important role in progression of colorectal carcinoma and that PLD2 could be a target for therapy in colorectal carcinoma.