Fabrication of Robust, Shape Recoverable, Macroporous Bacterial Cellulose Scaffolds for Cartilage Tissue Engineering
Fabrication of Robust, Shape Recoverable, Macroporous Bacterial Cellulose Scaffolds for Cartilage Tissue Engineering
复制标题
用于软骨组织工程的坚固、形状可恢复的大孔细菌纤维素支架的制造。
DOI：
10.1002/mabi.202100167
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发表时间：
2021-09-08
影响因子：
4.6
通讯作者：
Luo, Honglin
中科院分区：
文献类型：
--
作者：
Xun, Xiaowei;Li, Yaqiang;Luo, Honglin
近年来，利用机械破碎的细菌纤维素（MP-BC）片段制备三维大孔支架引起了人们的广泛关注。然而，这些材料的成功实施主要取决于它们的机械稳定性和坚固性。在此，使用无毒交联剂1-乙基-3-（-3-二甲基氨基丙基）碳二亚胺盐酸盐/N-羟基琥珀酰亚胺（EDC/NHS）来诱导BC片段之间的交联反应。除了它们的大孔径之外，由于BC纳米纤维上的EDC/NHS诱导的交联，EDC/NHS交联的MP-BC支架表现出优异的压缩性能和形状恢复能力。体外研究结果表明，MP-BC支架的生物相容性优于原始BC支架，因为前者为细胞增殖提供了更多的空间。体内实验结果表明，在裸鼠体内成功地再生了具有天然软骨外观和丰富的软骨特异性细胞外基质沉积的新软骨组织。这些发现揭示了具有可控孔径和形状可恢复特性的机械坚固的BC支架在组织工程中的巨大应用潜力。
Recently, the fabricating of three-dimensional (3D) macroporous bacterial cellulose (MP-BC) scaffolds with mechanically disintegrated BC fragments has attracted considerable attention. However, the successful implementation of these materials depends mainly on their mechanical stability and robustness. Here, a non-toxic crosslinker, 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide hydrochloride/N-hydroxysuccinimide (EDC/NHS), is employed to induce crosslinking reactions between BC fragments. In addition to their large pore sizes, the EDC/NHS-crosslinked MP-BC scaffolds exhibit excellent compression properties and shape recovery ability, owing to the EDC/NHS-induced crosslinking on the BC nanofibers. The results of in vitro studies reveal that the biocompatibility of MP-BC scaffolds is better than that of pristine BC scaffolds because the former provided more space for cell proliferation. The results of in vivo studies show that the neocartilage tissue with native cartilage appearance and abundant cartilage-specific extracellular matrix deposition is successfully regenerated in nude mice. The findings reveal the immense application potential of mechanically robust BC scaffolds with controllable pore sizes and shape-recoverable properties in tissue engineering.