Association of Serum and Salivary Tumor Necrosis Factor-α with Histological Grading in Oral Cancer and its Role in Differentiating Premalignant and Malignant Oral Disease
Association of Serum and Salivary Tumor Necrosis Factor-α with Histological Grading in Oral Cancer and its Role in Differentiating Premalignant and Malignant Oral Disease
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DOI：
10.7314/apjcp.2014.15.17.7141
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Anandan, Nirmala
中科院分区：
文献类型：
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作者：
Krishnan, Rajkumar;Thayalan, Dinesh Kumar;Anandan, Nirmala
背景：口腔鳞状细胞癌（OSCC）是世界范围内的一种重要恶性肿瘤；早期发现是实现高治愈率的重要标准。在众多报道的 OSCC 标志物中，本研究验证了肿瘤坏死因子-α (TNF-α) 在鉴别诊断口腔癌前病变和 OSCC 方面的功效。此外，该研究旨在将唾液和血清 TNF-α 水平与临床病理因素相关联。材料和方法：设计了一项前瞻性实验实验室研究。根据适当的排除和纳入标准，收集健康对照、癌前疾病 (PMD) 和 OSCC 每组 100 名受试者的血清和唾液样本用于研究。通过酶联免疫吸附测定分析血清和唾液中的TNF-α水平。对获得的数据进行适当的统计分析。结果：与健康对照组和 PMD 组相比，在 OSCC 受试者中观察到血清和唾液 TNF-α 水平升高。受试者操作特征曲线分析和曲线下面积值显示唾液 TNF-α 在区分 OSCC 与 PMD 和健康对照方面具有高度特异性和敏感性。中、低分化病灶中TNF-α水平较高分化病灶及临床分期IV期显着升高。仅观察到 OSCC 和 TNF-α 的组织学分级呈正相关。结论：唾液 TNF-α 被证明对于检测 OSCC 具有优越性。 TNF-α 随组织学分级和临床分期的增加提示其在预后中的作用。
Background: Oral squamous cell carcinoma (OSCC) is an important malignancy throughout the world; early detection is an important criterion for achieving high cure rate. Out of the many reported markers for OSCC, this study validated the efficacy of tumor necrosis factor-alpha (TNF-alpha) in differentially diagnosing premalignant oral lesions and OSCC. Also, the study aimed to correlate the levels of salivary and serum TNF-alpha with clinicopathologic factors. Materials and Methods: A prospective experimental laboratory study was designed. Serum and salivary samples from 100 subjects in each group of healthy control, premalignant disease (PMD) and OSCC were collected for the study following appropriate exclusion and inclusion criteria. Serum and salivary level of TNF-alpha was analysed by enzyme linked immunosorbent assay. The data obtained were subjected to appropriate statistical analysis. Results: Increased level of both serum and salivary TNF-alpha was observed in OSCC subjects compared to healthy control and PMD group. Receiver operator characteristic curve analysis and area under curve values showed high specificity and sensitivity for salivary TNF-alpha in differentiating OSCC from PMD and healthy controls. There was significant increase in TNF-alpha level in moderately and poorly differentiated lesion compared to well differentiated lesion and in stage IV of clinical stage. A positive correlation was observed only with histological grading of OSCC and TNF-alpha. Conclusions: Salivary TNF-alpha is proved to be superior for detecting OSCC. Increase in TNF-alpha with histological grading and clinical staging suggests a role in prognosis.