The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis
The interaction of CaM7 and CNGC14 regulates root hair growth in Arabidopsis
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CaM7 和 CNGC14 的相互作用调节拟南芥根毛生长
DOI：
10.1111/jipb.12890
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发表时间：
2020
影响因子：
11.4
通讯作者：
Liu Liangyu
中科院分区：
文献类型：
--
作者：
Zeb Qudsia;Wang Xiaohan;Hou Congcong;Zhang Xiwen;Dong Mengqi;Zhang Sisi;Zhang Qian;Ren Zhijie;Tian Wang;Zhu Huifen;Li Legong;Liu Liangyu
细胞质游离钙的振荡决定了根尖生长根毛的极性。Ca ~（2+）通道环核苷酸门控通道14（CNGC 14）参与了根尖Ca ~（2+）浓度梯度的动态变化。然而，调控CNGC 14的机制尚不清楚。在这项研究中，我们通过酵母双杂交和双分子荧光互补试验检测了钙调素7（CaM 7）和CNGC 14之间的直接相互作用。我们证明了CaM 7的第三个EF手结构域与CNGC 14的胞质C末端结构域特异性相互作用。双电极电压钳试验表明，CaM 7完全抑制CNGC 14介导的Ca 2+内流，表明CaM 7负调节CNGC 14介导的钙信号传导。此外，CaM 7过表达株系复制了acngc 14突变体的短根毛表型，并且该表型对外界Ca 2+浓度的变化不敏感。因此，我们将CaM 7-CNGC 14确定为一种新的相互作用模块，通过控制尖端聚焦的Ca 2+信号来调节根毛的极性生长。
Oscillations in cytosolic free calcium determine the polarity of tip‐growing root hairs. The Ca2+channel cyclic nucleotide gated channel 14 (CNGC14) contributes to the dynamic changes in Ca2+concentration gradient at the root hair tip. However, the mechanisms that regulate CNGC14 are unknown. In this study, we detected a direct interaction between calmodulin 7 (CaM7) and CNGC14 through yeast two‐hybrid and bimolecular fluorescence complementation assays. We demonstrated that the third EF‐hand domain of CaM7 specifically interacts with the cytosolic C‐terminal domain of CNGC14. A two‐electrode voltage clamp assay showed that CaM7 completely inhibits CNGC14‐mediated Ca2+influx, suggesting that CaM7 negatively regulates CNGC14‐mediated calcium signaling. Furthermore,CaM7overexpressing lines phenocopy the short root hair phenotype of acngc14mutant and this phenotype is insensitive to changes in external Ca2+concentrations. We, thus, identified CaM7‐CNGC14 as a novel interacting module that regulates polar growth in root hairs by controlling the tip‐focused Ca2+signal.