Evaluation of a new magnetic bead as an integrated platform for systematic CTC recognition, capture and clinical analysis
Evaluation of a new magnetic bead as an integrated platform for systematic CTC recognition, capture and clinical analysis
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对新型磁珠作为系统 CTC 识别、捕获和临床分析集成平台的评估
DOI：
10.1016/j.colsurfb.2020.111542
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发表时间：
2021-03-01
影响因子：
5.8
通讯作者：
Li, Peiyong
中科院分区：
文献类型：
--
作者：
Lu, Xinmiao;Tan, Sheng;Li, Peiyong
通过将Fe3O4纳米粒子嵌入两亲性抗体修饰的脂质体中，制备了一种新型的磁珠，即抗体包裹的磁性脂质纳米载体(AMLV)，作为高效的循环肿瘤细胞(CTC)捕捉剂。基于扩大的患者样本(包括318例结直肠癌患者、78例乳腺癌患者、77例肺癌患者和55例肝癌患者)，验证了AMLV的CTC捕获性能。并与CellSearch进行了初步比较，结果表明，细胞膜表面AMLV(EpCAM)对外周血中不同类型EpCAM表达的循环肿瘤细胞具有更高的捕获性能(AMLV(EpCAM)比CellSearch TM，n=5，P
A novel form of magnetic bead, namely antibody-coated magnetic lipid nano-vehicle (AMLV), was synthesized by embedding Fe3O4 nanoparticles into an amphiphilic antibody-modified liposome as a high-performance circulating tumor cell (CTC) hunter. The CTC capture performance of AMLV was validated based on an enlarged patient sample (including 318 colorectal, 78 breast, 77 lung and 55 liver cancer patients) with high detection rate. The preliminary comparison with Cellsearch was also conducted, indicating that the cell membrane-semblance AMLV(EpCAM) showed higher capture performance for different kinds of EpCAM-expressed circulating tumor cells in the peripheral blood (4.4 +/- 1.2-fold for AMLV(EpCAM) vs CellsearchTM, n=5, P