Does Japanese encephalitis virus share the same cellular receptor with other mosquito-borne flaviviruses on the C6/36 mosquito cells?
Does Japanese encephalitis virus share the same cellular receptor with other mosquito-borne flaviviruses on the C6/36 mosquito cells?
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DOI：
10.1186/1743-422x-4-83
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发表时间：
2007-09-06
期刊：
影响因子：
4.8
通讯作者：
Ma W
中科院分区：
文献类型：
--
作者：
Ren J;Ding T;Zhang W;Song J;Ma W
日本脑炎病毒（Japanese encephalitis virus，JEV）是蚊媒黄病毒科（Flaviviridae）的成员。迄今为止，JEV感染早期事件的机制仍然知之甚少，细胞受体也未被鉴定。有证据表明，蚊媒黄病毒的病毒附着蛋白（VAP）、囊膜糖蛋白的结构非常相似，蚊媒黄病毒的载体-病毒相互作用也非常相似。基于先前研究表明存在于蚊子细胞上的类似分子参与JEV、西尼罗河病毒（WNV）和登革病毒（DV）的摄取过程，提出蚊媒黄病毒（JEV、WNV和DV）的相同受体分子可能存在于C6/36蚊子细胞表面。通过免疫共沉淀法，我们研究了C6/36细胞上一个74 KDa的蛋白与JEV结合，质谱分析结果表明它可能是埃及伊蚊热休克同源蛋白70（HSC 70）。基于其他病毒使用热休克蛋白70（HSP 70）家族蛋白作为细胞受体，其可能参与JEV E蛋白与C6/36细胞膜的融合，以及已知形式的阳离子通道参与HSC 70与脂质双层的相互作用，进一步提出HSC 70作为穿透受体介导JEV进入C6/36细胞。
Japanese encephalitis virus (JEV) is a member of mosquito-borne Flaviviridae. To date, the mechanisms of the early events of JEV infection remain poorly understood, and the cellular receptors are unidentified. There are evidences that the structure of the virus attachment proteins (VAP), envelope glycoprotein of mosquito-borne flaviviruses is very similar, and the vector-virus interaction of mosquito-borne flaviviruses is also very similar. Based on the studies previously demonstrated that the similar molecules present on the mosquito cells involved in the uptake process of JEV, West Nile virus (WNV) and Dengue virus (DV), it is proposed that the same receptor molecules for mosquito-borne flaviviruses (JEV, WNV and DV) may present on the surface of C6/36 mosquito cells. By co-immunoprecipitation assay, we investigated a 74-KDa protein on the C6/36 cells binds JEV, and the mass spectrometry results indicated it may be heat shock cognate protein 70(HSC70) from Aedes aegypti. Based upon some other viruses use of heat shock protein 70 (HSP70) family proteins as cell receptors, its possible HSC70's involvement in the fusion of the JEV E protein with the C6/36 cells membrane, and known form of cation channels in the interaction of HSC70 with the lipid bilayer, it will further be proposed that HSC70 as a penetration receptor mediates JEV entry into C6/36 cells.