Introducing chemiluminescence resonance energy transfer into immunoassay in a microfluidic format for an improved assay sensitivity.
Introducing chemiluminescence resonance energy transfer into immunoassay in a microfluidic format for an improved assay sensitivity.
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DOI：
10.1039/c1cc16435j
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发表时间：
2012-01
影响因子：
4.9
通讯作者：
Shulin Zhao;Jinwen Liu;Yong Huang;Yi-Ming Liu
中科院分区：
文献类型：
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作者：
Shulin Zhao;Jinwen Liu;Yong Huang;Yi-Ming Liu
我们报道了一种通过在免疫反应中引入化学发光共振能量转移（CRET）来提高微流控免疫分析灵敏度的新策略。提出的基于cret的雌二醇（E2，作为模型分析物）免疫分析法是最敏感的免疫分析法之一，在微流控格式下检测限为3.6 × 10(-11) M E2。
We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with a limit of detection at 3.6 × 10(-11) M E2 in a microfluidic format.