High-density epicardial mapping during current injection and ventricular activation in rat hearts
High-density epicardial mapping during current injection and ventricular activation in rat hearts
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DOI：
10.1152/ajpheart.1998.275.5.h1886
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发表时间：
1998-11-01
影响因子：
4.8
通讯作者：
Taccardi, B
中科院分区：
文献类型：
--
作者：
Macchi, E;Cavalieri, M;Taccardi, B
本研究的目的是报告制造精密电极阵列的新方法，用于记录小动物心脏心外膜表面的高分辨率电位分布。构建了64导联（8×8）和121导联（11×11）的电极阵列，其基底为薄纱，通过在540×720μm的网眼节点间隔处打结，将绝缘的细银丝（直径60μm）附着在基底上。在结的末端去除绝缘层。在心室起搏搏动期间以及在舒张间期被动电流注入期间，从生理盐水溶液和大鼠心脏心外膜记录电位分布和波形。从大鼠心外膜获得的电反应与在大型动物心脏研究中观察到的结果相比具有优势，大型动物心脏研究使用的电极间距更大的阵列，并揭示了心肌各向异性的影响。在起搏部位周围区域刺激后早期测量的心外膜电位根据等效四极源产生的电位进行解释。我们得出结论，用于小型心脏心外膜标测的电极阵列可以足够容易和精确地构建，以便在正常和病理条件下详细研究这些心脏的纤维结构和电生理学。
The purpose of this study is to report new methods for manufacturing precision electrode arrays for recording high-resolution potential distributions from epicardial surfaces of small-animal hearts. Electrode arrays of 64 leads (8 x 8) and 121 leads (11 x 11) were constructed with a tulle substrate to which insulated, fine silver wires (60-mu m diameter) were attached by knots at mesh node intervals of 540 x 720 mu m. Insulation was removed at the tips of the knots. Potential distributions and waveforms were recorded from saline solutions and rat heart epicardium during ventricular paced beats and during passive current injection in the diastolic interval. Electrical responses obtained from rat epicardium compared favorably with those observed in studies of larger-animal hearts, which used arrays having greater electrode spacing, and revealed the effects of myocardial anisotropy. Epicardial potentials measured early after stimulation in the region surrounding the pacing site were interpreted in terms of potentials generated by an equivalent quadrupolar source. We conclude that electrode arrays for epicardial mapping of small hearts can be constructed with sufficient ease and precision to allow detailed study of fiber structure and electrophysiology in these hearts in normal and pathological conditions.