Based serum metabolomics analysis reveals simultaneous interconnecting changes during chicken embryonic development
Based serum metabolomics analysis reveals simultaneous interconnecting changes during chicken embryonic development
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基于血清代谢组学的分析揭示了鸡胚胎发育过程中同时发生的相互关联的变化
DOI：
10.1111/jpn.12925
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发表时间：
2018-10-01
影响因子：
2.7
通讯作者：
Ma, H. T.
中科院分区：
文献类型：
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作者：
Peng, M. L.;Li, S. N.;Ma, H. T.
代谢紊乱是一个主要的健康问题，与许多代谢疾病有关。由于鸡的高脂血症和对外源性胰岛素的抵抗，鸡作为脂肪组织生物学、代谢和肥胖研究的模型已被广泛应用。但鸡胚胎发育不同阶段血清代谢物的综合变化尚缺乏详细的资料。本研究采用LC/MS-QTOF法测定了孵化14 d（E14 d）、19 d（E19 d）和孵化1 d（H1 d）鸡胚胎发育过程中主要功能代谢产物的变化，并采用代谢物集富集分析法（Metabolite Set Enrichment Analysis）分析了鸡胚胎发育过程中差异代谢产物的相关途径。结果表明，从E14 d到E19 d，鸡胚中有39种代谢产物发生了显著变化，从E19 d到H1 d，有68种代谢产物发生了显著变化。E14 ~ E19 d，随着L-谷氨酰胺和苏氨酸浓度的增加，蛋白质合成得到促进，雌酮含量增加，性腺发育得到促进，表明血清谷氨酰胺、苏氨酸和雌酮含量可作为评价早期胚胎发育的候选指标。2-氧代谷氨酸主要促进柠檬酸循环，在鸡胚发育后期对鸡胚的生长有重要的促进作用; L-谷氨酰胺、L-异亮氨酸和L-亮氨酸在鸡胚发育过程中从E19 d到H1 d含量的降低，提示其可能作为肉鸡早期饲料添加剂，满足鸡胚的生长。这些结果为了解血清代谢物在鸡胚不同发育阶段的作用提供了依据，也为鸡作为代谢性疾病或人类肥胖研究的模型提供了有用的信息。
Metabolic disorder is a major health problem and is associated with a number of metabolic diseases. Due to native hyperglycaemia and resistance to exogenous insulin, chickens as a model had used in the studies of adipose tissue biology, metabolism and obesity. But no detailed information is available about the comprehensive changes of serum metabolites at different stages of chicken embryonic development. This study employed LC/MS-QTOF to determine the changes of major functional metabolites at incubation day 14 (E14d), 19 (E19d) and hatching day 1 (H1d), and the associated pathways of differential metabolites during chicken embryonic development were analysed using Metabolite Set Enrichment Analysis method. Results showed that 39 metabolites were significantly changed from E14d to E19d and 68 metabolites were significantly altered from E19d to H1d in chicken embryos. Protein synthesis was promoted by increasing the concentrations of L-glutamine and threonine, and gonadal development was promoted through increasing oestrone content from E14d to E19d in chicken embryos, which indicated that serum glutamine, threonine and oestrone contents may be considered as the candidate indicators for assessment of early embryonic development. 2-oxoglutaric acid mainly contributed to enhancing the citric cycle, and it plays an important role in improving the growth of chicken embryos at the late development; the decreasing of L-glutamine, L-isoleucine and L-leucine contents from E19d to H1d in chicken embryonic development implied their possible functions as the feed additive during early posthatch period of broiler chickens to satisfy the growth. These results provided insights into understand the roles of serum metabolites at different developmental stages of chicken embryos, it also provides available information for chicken as a model to study metabolic disease or human obesity.