HIGH-RESOLUTION PAS STAIN FOR POLYACRYLAMIDE-GEL ELECTROPHORESIS
HIGH-RESOLUTION PAS STAIN FOR POLYACRYLAMIDE-GEL ELECTROPHORESIS
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DOI：
10.1016/0003-2697(73)90202-9
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发表时间：
1973-01-01
影响因子：
2.9
通讯作者：
ZEBROWSK.EJ
中科院分区：
文献类型：
--
作者：
KAPITANY, RA;ZEBROWSK.EJ
描述了一种改进的 PAS 方法，用于在丙烯酰胺或脲-丙烯酰胺凝胶上电泳后检测糖蛋白。更强的氧化条件和更受控的去除高碘酸的洗涤导致染色强度和条带分辨率的增加。该方法不会对蛋白质进行染色，并且似乎可以轻松检测到 2-3 μg 结合或可沉淀碳水化合物的样品。通过该方法未检测到粘多糖。
An improved PAS method for the detection of glycoproteins after electrophoresis on acrylamide or urea-acrylamide gels is described. Stronger oxidizing conditions and a more controlled washing for the removal of periodic acid resulted in both increased staining intensity and band resolution. The method will not stain protein and it appears that a 2–3 μg sample of bound or precipitable carbohydrate would easily be detected. Mucopolysaccharides were not detectable by this method.