Rad54p is a chromatin remodeling enzyme required for heteroduplex DNA joint formation with chromatin
Rad54p is a chromatin remodeling enzyme required for heteroduplex DNA joint formation with chromatin
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DOI：
10.1074/jbc.m211545200
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发表时间：
2003-03-14
影响因子：
4.8
通讯作者：
Peterson, CL
中科院分区：
文献类型：
--
作者：
Jaskelioff, M;Van Komen, S;Peterson, CL
In eukaryotic cells, the repair of DNA double-strand breaks by homologous recombination requires a RecA-like recombinase, Rad51p, and a Swi2p/Snf2p-like ATPase, Rad54p. Here we find that yeast Rad51p and Rad54p support robust homologous pairing between single-stranded DNA and a chromatin donor. In contrast, bacterial RecA is incapable of catalyzing homologous pairing with a chromatin donor. We also show that Rad54p possesses many of the biochemical properties of bona fide ATP-dependent chromatin-remodeling enzymes, such as ySWI/SNF. Rad54p can enhance the accessibility of DNA within nucleosomal arrays, but it does not seem to disrupt nucleosome positioning. Taken together, our results indicate that Rad54p is a chromatin-remodeling enzyme that promotes homologous DNA pairing events within the context of chromatin.
