Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome
Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome
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DOI：
10.1073/pnas.1012891107
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发表时间：
2010-10-12
影响因子：
11.1
通讯作者：
Weigel, Detlef
中科院分区：
文献类型：
--
作者：
Laubinger, Sascha;Zeller, Georg;Weigel, Detlef
在拟南芥中，四种不同的切丁机样 (DCL) 蛋白在来自较长非编码前体 RNA 的 microRNA (miRNA) 和 siRNA 的生物合成中具有不同但部分重叠的功能。为了分析小RNA生物发生机制的不同组成部分对转录组的影响，我们对dcl和其他小RNA生物发生受损的突变体进行了全基因组平铺阵列分析。我们比较了两种组织和几种应激条件下的蛋白质编码基因和非编码转录本，包括大多数 pri-miRNA。我们的分析揭示了 dcl1 和 dcl2 dcl3 dcl4 三重突变体中数量惊人的共同靶点。此外，我们的结果表明，DCL1 不仅参与 miRNA 的作用，而且还有助于转座子子集的沉默，显然是通过对 DNA 甲基化的影响。
In Arabidopsis thaliana, four different dicer-like (DCL) proteins have distinct but partially overlapping functions in the biogenesis of microRNAs (miRNAs) and siRNAs from longer, noncoding precursor RNAs. To analyze the impact of different components of the small RNA biogenesis machinery on the transcriptome, we subjected dcl and other mutants impaired in small RNA biogenesis to whole-genome tiling array analysis. We compared both protein-coding genes and noncoding transcripts, including most pri-miRNAs, in two tissues and several stress conditions. Our analysis revealed a surprising number of common targets in dcl1 and dcl2 dcl3 dcl4 triple mutants. Furthermore, our results suggest that the DCL1 is not only involved in miRNA action but also contributes to silencing of a subset of transposons, apparently through an effect on DNA methylation.