Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair.
Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair.
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DOI：
10.1016/j.xpro.2023.102470
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发表时间：
2023-09-15
期刊：
影响因子：
--
通讯作者：
Isaac, H.
中科院分区：
文献类型：
--
作者：
Jgamadze, Dennis;Harary, Paul M.;Castellanos, Mackenzie;Blue, Rachel;Song, Hongjun;Ming, Guo-li;Isaac, H.
人类干细胞衍生的类器官是基于移植的神经修复的一个有前途的基质。在这里，我们描述了一种将前脑类器官移植到受伤的成年大鼠视觉皮层的方案。该方案包括颅骨切除术、误吸损伤、类器官移植和颅骨成形术的手术细节。该平台为研究类器官作为神经修复结构移植物的功效提供了一个有价值的工具。关于该协议的使用和执行的完整细节，请参考Jgamadze等人。用于移植的类脑器官的制备在成年大鼠皮层中创建可重复的抽吸腔的优化方案将人类前脑类器官移植到损伤腔的分步指南出版商注：开展任何实验方案都需要遵守当地机构的实验室安全和伦理指导方针。人类干细胞衍生的类器官是基于移植的神经修复的一个有前途的基质。在这里，我们描述了一种将前脑类器官移植到受伤的成年大鼠视觉皮层的方案。该方案包括颅骨切除术、误吸损伤、类器官移植和颅骨成形术的手术细节。该平台为研究类器官作为神经修复结构移植物的功效提供了一个有价值的工具。
Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair. For complete details on the use and execution of this protocol, please refer to Jgamadze et al. Preparation of brain organoids for transplantation Optimized protocol for creating a reproducible aspiration cavity in an adult rat cortex Step-by-step guide for transplanting human forebrain organoids into an injury cavity Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair.
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DOI：
10.3791/3564
发表时间：
2012-07-30
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Gage, Gregory J;Kipke, Daryl R;Shain, William
通讯作者：
Shain, William
影响因子：
23.9
作者：
Jgamadze, Dennis;Lim, James T.;Chen, Han-Chiao Isaac
通讯作者：
Chen, Han-Chiao Isaac
影响因子：
64.5
作者：
Qian X;Nguyen HN;Song MM;Hadiono C;Ogden SC;Hammack C;Yao B;Hamersky GR;Jacob F;Zhong C;Yoon KJ;Jeang W;Lin L;Li Y;Thakor J;Berg DA;Zhang C;Kang E;Chickering M;Nauen D;Ho CY;Wen Z;Christian KM;Shi PY;Maher BJ;Wu H;Jin P;Tang H;Song H;Ming GL
通讯作者：
Ming GL
影响因子：
64.8
作者：
Wen Z;Nguyen HN;Guo Z;Lalli MA;Wang X;Su Y;Kim NS;Yoon KJ;Shin J;Zhang C;Makri G;Nauen D;Yu H;Guzman E;Chiang CH;Yoritomo N;Kaibuchi K;Zou J;Christian KM;Cheng L;Ross CA;Margolis RL;Chen G;Kosik KS;Song H;Ming GL
通讯作者：
Ming GL
影响因子：
10.9
作者：
Routt, Ethan;Mansouri, Yasaman;Levitt, Jacob
通讯作者：
Levitt, Jacob