Complete life cycle of Myxobolus rotundus (Myxosporea: Myxobolidae), a gill myxozoan of common bream Abramis brama.
Complete life cycle of Myxobolus rotundus (Myxosporea: Myxobolidae), a gill myxozoan of common bream Abramis brama.
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DOI：
10.3354/dao02068
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发表时间：
2009-06
影响因子：
1.4
通讯作者：
C. Székely;S. Hallett;S. Atkinson;K. Molnár
中科院分区：
文献类型：
--
作者：
C. Székely;S. Hallett;S. Atkinson;K. Molnár
在实验室条件下，研究了白鲷鳃黏液孢子寄生虫圆形粘虫（Myxobolus rotundus Nemeczek, 1911）的生活史。利用野生鲷鱼鳃中疟原虫的成熟黏液孢子感染水族流动系统中的naïve寡毛虫。三放线菌型放线菌孢子在1年后从寡毛菌中释放出来，并不断流入装有未感染的鲷鱼苗的水箱中。暴露后第3天检查鱼苗鳃中疟原虫的发展情况，然后每周检查一次，持续8周。在每个时间点固定组织样本。暴露后17 d（第17天）首次观察到正在发育的疟原虫。在第56天从疟原虫中收集成熟孢子，并将其加入含有无寄生虫的管fex Tubifex oligochetes的塑料培养皿中。第二代放线菌孢子在暴露后8个月从这些蠕虫中释放出来，并且在形态上与第一代孢子相同。从鲷鱼苗中获得的黏液孢子在形态上与在野生鲷中鉴定的圆形芽孢杆菌相同。从第一代和第二代放线菌孢子和鲷苗黏液孢子中获得的小亚基核糖体RNA基因序列与原始野生鱼类的圆形芽孢杆菌孢子100%相似。
The life cycle of Myxobolus rotundus Nemeczek, 1911, a myxosporean parasite of the gills of common bream Abramis brama L., was studied under laboratory conditions. Mature Myxobolus spp. spores from plasmodia in the gills of wild bream were used to infect naïve oligochaete worms in a flow-through system of aquaria. Triactinomyxon-type actinospores were released from the oligochaetes 1 yr later and allowed to continually flow into a tank containing uninfected bream fry. The gills of the fry were checked for development of plasmodia in squash preparations 3 d postexposure, and then at weekly intervals for 8 wk. Tissue samples were fixed at each time point. Developing plasmodia were first observed 17 d post-exposure (Day 17). Mature spores were collected from plasmodia on Day 56 and were added to plastic dishes containing parasite-free Tubifex tubifex oligochaetes. Second-generation actinospores were released from these worms 8 mo post-exposure, and were morphologically identical to first-generation spores. Myxospores obtained from the bream fry were morphologically identical to those identified in wild bream as M. rotundus. Small subunit ribosomal RNA gene sequences obtained from first- and second-generation actinospores and the bream fry myxospores were 100% similar to M. rotundus spores from the original wild fish.