Plasma jet-induced tissue oxygenation: potentialities for new therapeutic strategies
Plasma jet-induced tissue oxygenation: potentialities for new therapeutic strategies
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DOI：
10.1088/0963-0252/23/1/012005
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发表时间：
2014-02-01
影响因子：
3.8
通讯作者：
Pouvesle, J. M.
中科院分区：
文献类型：
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作者：
Collet, G.;Robert, E.;Pouvesle, J. M.
缺氧是肿瘤细胞对放射疗法等治疗产生抗性的主要原因。大量的非热等离子体在医学中的应用报告细胞行为的修改和可溶性因子的调制的最新出版物。该体内研究测试了这种修饰是否会导致血管对血浆应用的响应变化。同时使用两种基于光学的原位方法，在真实的时间，以评估非热等离子体对组织脉管系统的影响。组织氧分压（pO（2））的测量使用时间分辨发光为基础的光学探头，微血管红细胞流量测定激光多普勒血流仪。当将血浆处理应用于小鼠皮肤时，皮下测量到pO（2）快速增加（高达4倍），并与血流改善相关。这种短的持续时间，即5分钟，等离子体诱导的效果被证明是局部限制到治疗的区域，并持续超过120分钟。进一步的调查应阐明这些过程的分子机制。然而，氧合和灌注的改善为肿瘤治疗与放疗的组合以及基于肿瘤血管正常化的策略打开了新的机会。
The lack of oxygen is a major reason for the resistance of tumor cells to treatments such as radiotherapies. A large number of recent publications on non-thermal plasma applications in medicine report cell behavior modifications and modulation of soluble factors. This in vivo study tested whether such modifications can lead to vascular changes in response to plasma application. Two in situ optical-based methods were used simultaneously, in real time, to assess the effect of non-thermal plasma on tissue vasculature. Tissue oxygen partial pressure (pO(2)) was measured using a time-resolved luminescence-based optical probe, and the microvascular erythrocyte flow was determined by laser Doppler flowmetry. When plasma treatment was applied on mouse skin, a rapid pO(2) increase (up to 4 times) was subcutaneously measured and correlated with blood flow improvement. Such short duration, i.e. 5 min, plasma-induced effects were shown to be locally restricted to the treated area and lasted over 120 min. Further investigations should elucidate the molecular mechanisms of these processes. However, improvement of oxygenation and perfusion open new opportunities for tumor treatments in combination with radiotherapy, and for tumor blood vessel normalization based strategies.