Bacterial community shifts evaluation in the sediments of Puyang River and its nitrogen removal capabilities exploration by resuscitation promoting factor
Bacterial community shifts evaluation in the sediments of Puyang River and its nitrogen removal capabilities exploration by resuscitation promoting factor
复制标题
濮阳河沉积物细菌群落变化评价及复苏促进因子脱氮能力探讨
DOI：
10.1016/j.ecoenv.2019.04.067
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发表时间：
2019-09-15
影响因子：
6.8
通讯作者：
Sun, Faqian
中科院分区：
文献类型：
--
作者：
Su, Xiaomei;Xue, Binbing;Sun, Faqian
识别原生细菌群落和发掘原生微生物的潜力对于水体中含氮污染物的原位生物修复至关重要。本研究评估了氮污染河流沉积物样品中的细菌群落，并揭示了可能的环境因素对细菌种群的影响。重要的是，利用复苏促进因子(RPF)探索了在沉积物土著种群中具有脱氮能力的活的但不可培养的细菌。结果表明，浦阳河上游(URS)和下游(LRS)底泥具有不同的污染物水平和细菌群落。硝态氮、有机碳和氨氮可能对土壤细菌组成有显著影响。从URS和LRS中，通过添加RPF对13株具有异养硝化能力的菌株进行了复苏，它们分别属于芽孢杆菌属、假单胞菌属、营养狭窄性单胞菌属和不动杆菌属。其中，假单胞菌属(Pseudomonas sp.)结果表明，SSPR1具有较高的同步硝化反硝化能力，对氨氮和硝酸盐的平均去除速率分别为2.23 mg/(L.h)和0.86 mg/(L.h)。这些复活菌株可用于河流及其受纳水体的生物脱氮。
Identifying indigenous bacterial community and exploring the potential of native microorganisms are crucial for in situ bioremediation of nitrogenous pollutants in water bodies. This study evaluated the bacterial communities of sediment samples from a nitrogen polluted river, and revealed the possible environmental factors shaping the bacterial populations. Importantly, viable but non-culturable bacteria which possessed nitrogen removal capabilities in indigenous population of the sediments were explored by resuscitation promoting factor (Rpf). It was found that the sediments from upstream (URS) and lower stream (LRS) of Puyang river showed both different pollutants levels and bacterial community. Nitrate nitrogen, organic carbon and ammonium nitrogen probably had a significant effect on bacterial compositions between URS and LRS. From URS and LRS, a total of thirteen strains with heterotrophic nitrification ability were resuscitated by Rpf addition, which belonged to genera Bacillus, Pseudomonas, Stenotrophomonas and Acinetobacter. Among them, the strain Pseudomonas sp. SSPR1 was found to display high removal capabilities of simultaneous nitrification and denitrification, and the average ammonium and nitrate removal rates were 2.23 and 0.86 mg/(L.h), respectively. These resuscitated strains could be considered to be used for biological nitrogen removal in rivers and their receiving water bodies.