Identification of molecules preferentially expressed beneath the marginal zone in the developing cerebral cortex
Identification of molecules preferentially expressed beneath the marginal zone in the developing cerebral cortex
复制标题
DOI：
10.1016/j.neures.2007.10.006
复制
发表时间：
2008-02-01
影响因子：
2.9
通讯作者：
Nakajima, Kazunori
中科院分区：
文献类型：
--
作者：
Tachikawa, Kashiko;Sasaki, Shinji;Nakajima, Kazunori
在大脑皮层发育过程中，大多数兴奋性神经元出生在脑室附近，并向边缘区（MZ）径向迁移。由于细胞总是在MZ下停止迁移，神经元在皮质板（CP）中以“内向外”的方式排列；也就是说，早生和晚生的神经元最终分别位于深层和浅层。由于MZ以下的细胞发生了剧烈的形态变化，因此必须发生一些对神经元成熟和层形成至关重要的事件。在这项研究中，我们筛选了MZ下强烈表达的分子，并鉴定出28个基因在胚胎日(E) 16.5和E 18.5时优先表达在小鼠CP的上半部分。对reeler和yotari小鼠的表达分析表明，这些基因确实与MZ下的事件有关，而不是由靠近大脑表面的结构诱导的无关的事件。通路分析表明钙信号在MZ下的细胞中起重要作用。这里提出的基因列表将有助于阐明控制皮质发育的分子机制。(C) 2007爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
During cerebral cortical development, the majority of excitatory neurons are born near the ventricle and migrate radially toward the marginal zone (MZ). Since the cells invariably stop migrating beneath the MZ, neurons are aligned in an "inside-out" manner in the cortical plate (CP); that is, the early-born and late-born neurons are ultimately positioned in the deep and superficial layers, respectively. Since dramatic morphological changes occur in cells beneath the MZ, several events critical for proper neuronal maturation and layer formation must take place. In this study, we screened for molecules strongly expressed beneath the MZ, and identified 28 genes that are preferentially expressed in the upper half of the mouse CP on both embryonic day (E) 16.5 and E 18.5. Expression analyses in reeler and yotari mice, in which neurons terminate migration throughout the CP, suggested that these genes were indeed related to the events beneath the MZ rather than unrelatedly induced by the structures near the brain surface. Pathway analyses suggested calcium signaling to have an important role in cells beneath the MZ. The gene list presented here will be useful for clarifying the molecular mechanisms that control cortical development. (C) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.