Expression of leukemia inhibitory factor is regulated in human mesenchymal cells.
Expression of leukemia inhibitory factor is regulated in human mesenchymal cells.
复制标题
白血病抑制因子的表达在人间充质细胞中受到调节。
作者：
Michael Lübbert;L. Mantovani;Albrecht Lindemann;R. Mertelsmann;François Herrmann
对DA细胞有不同名称的白血病抑制因子（LIF）、分化诱导因子、分化抑制活性或人白细胞介素的蛋白质可以控制造血细胞以及几种其它细胞谱系的分化和增殖。为了进一步阐明LIF产生细胞的谱，我们使用北方印迹分析检测了不同细胞类型的LIF mRNA表达。在活化的正常人T细胞和两种T细胞系中检测到LIF mRNA，但在B淋巴细胞系、静息和活化的正常人粒细胞和单核细胞以及人骨髓细胞系K562和HL-60中检测不到。在人肺成纤维细胞和人脐静脉内皮细胞中，LIF组成型表达，并且在用佛波醇酯TPA治疗后以及在两种即时反应细胞因子肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1-β存在下，LIF的积累以时间依赖性方式增加。我们的结论是，LIF的mRNA不仅表达的T细胞，但也在人类间充质细胞。LIF转录本在这些细胞中的表达是组成性的，并且可以通过佛波酯、TNF-α和IL-1-β显著增强。
A protein variously termed leukemia inhibitory factor (LIF), differentiation-inducing factor, differentiation inhibitory activity or human interleukin for DA cells can control the differentiation and proliferation of hematopoietic cells as well as of several other cellular lineages. In order to further elucidate the spectrum of LIF-producing cells, we examined different cell types for the expression of LIF mRNA using Northern blot analysis. LIF mRNA was detected in activated normal human T-cells and in two T-cell lines but was undetectable in a B-lymphoid cell line, in both resting and activated normal human granulocytes and monocytes and in human myeloid cell lines K562 and HL-60. In human lung fibroblasts and in human umbilical vein endothelial cells, LIF was constitutively expressed and its accumulation was increased in a time-dependent manner following treatment with the phorbol ester TPA and in the presence of the two immediate response cytokines tumor necrosis factor (TNF)-alpha and interleukin (IL)-1-beta. We conclude that mRNA for LIF is not only expressed by T-cells but also in human mesenchymal cells. Expression of LIF transcripts in these cells is constitutive and can be significantly enhanced by phorbol ester, TNF-alpha and IL-1-beta.