Multidrug resistance protein gene expression in Trichoplusia ni caterpillars
Multidrug resistance protein gene expression in Trichoplusia ni caterpillars
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DOI：
10.1111/imb.12003
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发表时间：
2013-02-01
影响因子：
2.6
通讯作者：
Donly, Cam
中科院分区：
文献类型：
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作者：
Simmons, Jason;D'Souza, Olivia;Donly, Cam
许多昆虫都表现出抗杀虫剂的表型。导致耐药的机制之一是多药耐药（MDR）转运蛋白的过度表达。在此，我们克隆了3个编码毛癣菌MDR蛋白的基因：trnMDR1、trnMDR2和trnMDR3。实时荧光定量PCR （Real-time quantitative PCR, qPCR）检测末龄大鼠整个神经系统、中肠和马氏小管中trnMDR mRNA的表达。倪毛毛虫。为了测试这些基因是否在化学胁迫下被上调，我们使用qPCR比较了未受化学胁迫的昆虫与喂食人工拟除虫菊酯（溴氰菊酯）的昆虫的trnMDR mRNA水平。仅在trnMDR2基因中检测到有限的增加，该基因是三个耐多药耐药基因中表达最弱的，这表明这种类型的多药耐药增加不是对溴氰菊酯暴露反应的重要组成部分。
Many insect species exhibit pesticide-resistant phenotypes. One of the mechanisms capable of contributing to resistance is the overexpression of multidrug resistance (MDR) transporter proteins. Here we describe the cloning of three genes encoding MDR proteins from Trichoplusia ni: trnMDR1, trnMDR2 and trnMDR3. Real-time quantitative PCR (qPCR) detected trnMDR mRNA in the whole nervous system, midgut and Malpighian tubules of final instar T.?ni caterpillars. To test whether these genes are upregulated in response to chemical challenge in this insect, qPCR was used to compare trnMDR mRNA levels in unchallenged insects with those of insects fed the synthetic pyrethroid, deltamethrin. Only limited increases were detected in a single gene, trnMDR2, which is the most weakly expressed of the three MDR genes, suggesting that increased multidrug resistance of this type is not a significant part of the response to deltamethrin exposure.