Correlation of cytotoxicity with element release from mercury- and gallium-based dental alloys in vitro.
Correlation of cytotoxicity with element release from mercury- and gallium-based dental alloys in vitro.
复制标题
体外汞基和镓基牙科合金的细胞毒性与元素释放的相关性。
DOI：
10.1016/0109-5641(94)90037-x
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Okabe,T
中科院分区：
文献类型：
--
作者：
Wataha,JC;Nakajima,H;Hanks,CT;Okabe,T
目标.采用体外筛选试验比较汞基和镓基牙体修复材料的细胞毒性和元素释放。该试验采用三次连续浸提样品至细胞培养基中，然后用于评价样品的细胞毒性和样品中元素的释放。通过将浸提液与Balb/c小鼠成纤维细胞接触24小时并测量细胞的琥珀酸脱氢酶活性来测量细胞毒性。通过原子吸收分光光度法测量元素的释放。结果。与聚四氟乙烯对照品相比，Tytin（Kerr）样品没有显示出细胞毒性。分散合金（约翰逊和约翰逊）是严重的细胞毒性最初时，锌的释放是最大的，但毒性较小的48和72小时之间的锌释放减少。镓合金GF（Tokuriki Honten）在8 h后具有中度细胞毒性，此后细胞毒性增加，这与该材料中Ga的大量和持续释放相关。当前研究的结果与这些材料的体内评估一致，并且使用连续浸提可用于确定这些材料的细胞毒性和元素释放趋势。
Objectives. Anin vitroscreening test was used to compare the cytotoxicity and elemental release from mercury- and gallium-based dental restorative materials.Methods. The test employed three sequential extractions of the samples into cell-culture medium which were then used to evaluate the cytotoxicity of the samples and the release of elements from the samples. Cytotoxicity was measured by placing the extract in contact with Balb/c mouse fibroblasts for 24 h and measuring the succinic dehydrogenase activity of the cells. The release of elements was measured by means of atomic absorption spectrophotometry.Results. Samples of Tytin (Kerr) showed no cytotoxicity compared to Teflon controls. Dispersalloy (Johnson and Johnson) was severely cytotoxic initially when Zn release was greatest, but was less toxic between 48 and 72 h as Zn release decreased. Gallium Alloy GF (Tokuriki Honten) was moderately cytotoxic after 8 h, and increased in cytotoxicity thereafter, which correlated with a substantial and persistent release of Ga from this material.Significance. The results of the current study concurred within vivoassessments of these materials, and the use of sequential extractions was useful in determining trends in the cytotoxicity and elemental release from these materials.
登录
查看更多内容
美国国家标准协会/美国牙科协会 1 号牙科汞合金规范附录。
DOI：
--
发表时间：
1980
期刊：
The Journal of the American Dental Association (1939)
影响因子：
--
作者：
Instruments;Equipment
通讯作者：
Equipment
镓与粉末金属的合金可作为牙科用汞合金的替代品。
DOI：
--
发表时间：
1956
期刊：
The Journal of the American Dental Association (1939)
影响因子：
--
作者：
Denton L. Smith;H. J. Caul
通讯作者：
H. J. Caul
影响因子：
2.5
作者：
T. Yamashita;K. Itoh;S. Wakumoto
通讯作者：
S. Wakumoto
软组织对镓合金和银汞合金植入物的反应。
DOI：
--
发表时间：
1966
期刊：
The Journal of the American Dental Association (1939)
影响因子：
--
作者：
H. W. Lyon;R. Waterstrat;G. Paffenbarger
通讯作者：
G. Paffenbarger
细胞培养中镓合金和汞齐的比较细胞毒性评价。
DOI：
--
发表时间：
1992
期刊：
影响因子：
--
作者：
M. Kaga
通讯作者：
M. Kaga