PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3
PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3
复制标题
内质网膜重塑形成的包涵体上的 PI4KB 促进人副流感病毒 3 型的复制
DOI：
10.1016/j.celrep.2019.10.052
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发表时间：
2019-11-19
期刊：
影响因子：
8.8
通讯作者：
Chen, Mingzhou
中科院分区：
文献类型：
--
作者：
Li, Zhifei;Guo, Dong;Chen, Mingzhou
许多正链RNA病毒重塑内膜以形成专门的复制细胞器。然而，关于负链RNA病毒是否利用细胞内膜进行复制的知识是有限的。在这里，我们表明，负链RNA病毒，人副流感病毒3型（HPIV 3），重塑内质网（ER）膜形成包涵体（IB），从而磷蛋白（P）的HPIV 3招募磷脂酰肌醇4-激酶β（PI 4KB）IB产生PI 4P，创造一个PI 4P富集的微环境，以促进HPIV 3复制。此外，我们发现人呼吸道合胞病毒（HRSV）也利用ER形成IB，并且这些IB也富含PI 4P。HRSV的核蛋白将PI 4KB募集到IB。这些结果表明，副粘病毒还利用宿主内膜形成IB，并且病毒蛋白质募集PI 4KB以使IB富集PI 4P以促进病毒复制。
Many positive-strand RNA viruses remodel the endomembrane to form specialized replication organelles. However, knowledge regarding whether negative-strand RNA viruses take advantage of intracellular membranes for replication is limited. Here we show that a negative-strand RNA virus, human parainfluenza virus type 3 (HPIV3), remodels the endoplasmic reticulum (ER) membrane to form inclusion bodies (IBs), whereby the phosphoprotein (P) of HPIV3 recruits phosphatidylinositol 4-kinase beta (PI4KB) to IBs to generate PI4P, creating a PI4P-enriched microenvironment to promote HPIV3 replication. In addition, we find that human respiratory syncytial virus (HRSV) also takes advantage of the ER to form IBs and that these IBs are also enriched with PI4P. The nucleoprotein of HRSV recruits PI4KB to IBs. These results suggest that paramyxo-viruses also exploit the host endomembrane to form IBs and that PI4KB is recruited by viral proteins to enrich IBs with PI4P to facilitate viral replication.