Expression of cadherins and alpha-catenin in primary epithelial tumors of the liver
Expression of cadherins and alpha-catenin in primary epithelial tumors of the liver
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DOI：
10.1053/gast.1996.v110.pm8613003
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发表时间：
1996-04-01
期刊：
影响因子：
29.4
通讯作者：
Feldmann, G
中科院分区：
文献类型：
--
作者：
Kozyraki, R;Scoazec, JY;Feldmann, G
背景和目标：钙粘蛋白及其相关分子，如α-连环蛋白，最近已被证明在上皮癌发生中发挥关键作用。研究方法：应用免疫组化和Western blotting方法检测10例正常肝组织、28例局灶性结节增生、9例肝细胞腺瘤、65例肝细胞癌和9例胆管细胞癌中E-cadherin、N-cadherin和α-catenin的表达。结果：在正常肝脏中，肝细胞表达E-钙粘蛋白和129千道尔顿的钙粘蛋白的抗N-钙粘蛋白抗体GC 4鉴定。α-连环蛋白表达水平低。胆管细胞仅表达E-钙粘蛋白，并显示高水平的α-连环蛋白。钙粘素和α-连环素的表达在局灶性结节性增生中得以保留。在肝细胞腺瘤中，钙粘蛋白和α-连环蛋白表达不均匀。在肝细胞癌中，钙粘蛋白和α-连环蛋白表达经常减少或缺失。钙粘蛋白表达的改变与肿瘤大小、组织分化程度低、包膜和血管浸润的发生相关。在胆管癌中，肿瘤细胞不稳定地表达E-钙粘蛋白和α-连环蛋白。结论：钙粘素和α-连环素在肝细胞腺瘤和原发性肝癌中表达改变较为常见。它们在肝细胞癌中的发生率具有预后意义。
Background & Aims: Cadherins and their associated molecules, such as alpha-catenin, have been shown recently to play a pivotal role in epithelial carcinogenesis. Methods: The expression of E-cadherin, N-cadherin, and alpha-catenin in 10 normal samples, 28 focal nodular hyperplasias, 9 liver cell adenomas, 65 hepatocellular carcinomas, and 9 cholangiocarcinomas was studied by immunohistochemistry and Western blotting. Results: In the normal liver, hepatocytes expressed E-cadherin and a 129-kilodalton cadherin identified by the anti-N-cadherin antibody GC4. The expression level of alpha-catenin was low. Bile duct cells expressed only E-cadherin and showed high levels of alpha-catenin. The expression of cadherins and alpha-catenin was preserved in focal nodular hyperplasia. In liver cell adenomas, cadherins and alpha-catenin were heterogeneously expressed. In hepatocellular carcinomas, cadherin and alpha-catenin expression was frequently reduced or absent. Alterations in cadherin expression correlated with large tumor size, low grade of histological differentiation, and occurrence of capsular and vascular invasion. In cholangiocarcinomas, neoplastic cells inconstantly expressed E-cadherin and alpha-catenin. Conclusions: Alterations of cadherin and alpha-catenin expression are frequent in liver cell adenomas and primary liver carcinomas. Their incidence in hepatocellular carcinomas is of prognostic significance.