Monte Carlo fluorescence microtomography.
Monte Carlo fluorescence microtomography.
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蒙特卡罗荧光显微断层扫描。
DOI：
10.1117/1.3596171
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发表时间：
2011
影响因子：
3.5
通讯作者：
Wang,Ge
中科院分区：
文献类型：
--
作者：
Cong,AlexanderX;Hofmann,MatthiasC;Cong,Wenxiang;Xu,Yong;Wang,Ge
荧光显微镜可以实时监测光学分子探针，用于疾病表征、药物开发和组织再生。然而，当生物样品厚度超过 1 毫米时，强烈的光散射会显着降低荧光显微镜的空间分辨率。在本文中，我们开发了一种荧光显微断层扫描技术，利用蒙特卡罗方法对厚生物样品中的荧光报告基因进行成像。该方法基于 10 正则化断层扫描模型，提供了一个出色的解决方案。我们对仿生组织支架的研究表明，所提出的方法能够准确可靠地定位和量化光学分子探针的分布。
Fluorescence microscopy allows real-time monitoring of optical molecular probes for disease characterization, drug development, and tissue regeneration. However, when a biological sample is thicker than 1 mm, intense scattering of light would significantly degrade the spatial resolution of fluorescence microscopy. In this paper, we develop a fluorescence microtomography technique that utilizes the Monte Carlo method to image fluorescence reporters in thick biological samples. This approach is based on anl0-regularized tomography model and provides an excellent solution. Our studies on biomimetic tissue scaffolds have demonstrated that the proposed approach is capable of localizing and quantifying the distribution of optical molecular probe accurately and reliably.