EVIDENCE THAT PULSATILE FOLLICLE-STIMULATING-HORMONE SECRETION IS INDEPENDENT OF ENDOGENOUS LUTEINIZING-HORMONE-RELEASING HORMONE
EVIDENCE THAT PULSATILE FOLLICLE-STIMULATING-HORMONE SECRETION IS INDEPENDENT OF ENDOGENOUS LUTEINIZING-HORMONE-RELEASING HORMONE
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DOI：
10.1210/endo-118-2-609
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发表时间：
1986-02-01
期刊：
影响因子：
4.8
通讯作者：
NEGROVILAR, A
中科院分区：
文献类型：
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作者：
CULLER, MD;NEGROVILAR, A
虽然 LHRH 可以刺激垂体释放 LH 和 FSH，但在许多情况下 LH 和 FSH 的分泌是不同的。我们实验室之前的研究表明，脉冲性 LH 和 FSH 分泌受性腺因素独立调节。因此，我们通过评估去势成年雄性大鼠被动 LHRH 免疫中和 LH 和 FSH 分泌的效果，重新审视了 LHRH 在调节脉动促性腺激素分泌中的作用。注射500μl绵羊抗LHRH血清编号。 772 (LHRH-AS) 注入 2 周的去势大鼠体内，导致平均血浆 LH 水平在 2 小时内抑制了 85%，并持续了 48 小时。然而，平均血浆 FSH 在 2 小时后仅降低了 19%，在 48 小时后仅降低了 59%。当2周去势大鼠插管后每10分钟放血一次，LH和FSH均以脉冲方式分泌。注射500μl LHRH-AS导致LH脉冲立即消除并且平均血浆LH在24小时内迅速降低。尽管平均血浆 FSH 水平显着降低，但以脉冲频率和振幅参数为特征的脉动 FSH 分泌不受 LHRH-AS 的影响。总的来说，结果表明，脉冲式 FSH 分泌是由不同于 LHRH 的单独因素调节的，但 LHRH 是维持升高的 FSH 水平所必需的。
Although LHRH can stimulate the release of both LH and FSH from the pituitary, there are a number of instances in which the secretion of LH and FSH are divergent. Previous studies from our laboratory have indicated that pulsatile LH and FSH secretion are independently regulated by gonadal factors. We have, therefore, reexamined the role of LHRH in regulating pulsatile gonadotropin secretion by evaluating the effect of passive LHRH immunoneutralization of LH and FSH secretion in castrate adult male rats. Injection of 500 .mu.l ovine anti-LHRH serum no. 772 (LHRH-AS) into 2-week castrate rats caused an 85% suppression of mean plasma LH levels by 2 h, which lasted through 48 h. Mean plasma FSH, however, was reduced by only 19% after 2 h and by only 59% after 48 h. When cannulated 2-week-castrate rats were bled every 10 min, both LH and FSH were secreted in a pulsatile manner. Injection of 500 .mu.l LHRH-AS caused an immediate abolishment of LH pulses and a rapid reduction in mean plasma LH through 24 h. Pulsatile FSH secretion, as characterized by the parameters of pulse frequency and amplitude, was unaffected by LHRH-AS, although mean plasma FSH levels were significantly reduced. Collectively, the results suggest that pulsatile FSH secretion is regulated by a separate factor(s) distinct from LHRH, but that LHRH is required for the maintenance of elevated FSH levels.