Polyphasic characterization of the bacterial community in an urban soil profile with in situ and culture-dependent methods
Polyphasic characterization of the bacterial community in an urban soil profile with in situ and culture-dependent methods
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DOI：
10.1016/j.apsoil.2005.05.003
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发表时间：
2006-03-01
影响因子：
4.8
通讯作者：
Szewzyk, U
中科院分区：
文献类型：
--
作者：
Braun, B;Böckelmann, U;Szewzyk, U
城市土壤中细菌群落的研究至今尚未深入。因此，从柏林市中心的城市公园蒂尔加滕的土壤样品取自15，30和90厘米深的剖面。细菌总数（4，6-二脒基-2-苯基吲哚（DAPI）计数）以及其生物量从表土到底土下降一个数量级。土壤质地变化剧烈，土壤含水量和有机质含量下降3-10倍。可培养细菌的数量（菌落形成单位= CFU）也随着土壤深度的增加而减少。扩增核糖体DNA限制性内切酶分析（ARDIOR）显示，在两个上层土壤中的最深的一层相比，类似的细菌群落。用EUB 338探针检测到的细菌细胞数占总细胞数的比例在三个土层中的差异在43%和35%之间。与探针活性计数法（PAC），这个数字可以增加到72%的总细胞计数在表土，而细胞的活性随深度的增加而下降。相对于总细胞计数（DAPI）α-变形菌和β-变形菌均匀分布在所有三个深度，而γ-变形菌下降的土壤剖面内。在BIOBLOOD系统中，我们观察到总的趋势是，利用不同底物的能力随着土壤深度的增加而降低，而一些底物，如吐温40和吐温80，可以被所有土壤深度的细菌利用。(C)2005 Elsevier B.V.保留所有权利。
Bacterial communities of urban soils have not been thoroughly investigated up to now. Therefore, soil samples from the urban park Tiergarten in the centre of Berlin were taken from a profile in 15, 30 and 90 cm depth. The total number of bacteria (4,6-diamidino-2-phenylindole (DAPI) counts) as well its biomass declined one order of magnitude from topsoil to subsoil. Soil texture changed comparably and water content and amount of organic matter dropped 3-10-fold. The number of culturable bacteria (colony forming units = CFU) also decreased with increasing soil depth. Amplified ribosomal DNA restriction analysis (ARDRA) revealed similar bacterial communities in the two upper soil layers in contrast to the deepest layer. The number of bacterial cells which were detected with probe EUB338 in relation to total cell counts differed between 43 and 35% in the three soil layers. With the probe active Count Method (PAC) this number could be increased up to 72% of total cell counts in topsoil whereas activation of cells declined with increasing depth. In relation to total cell counts (DAPI) alpha-Proteobacteria and beta-Proteobacteria are equally distributed in all three depths, whereas gamma-Proteobacteria declined within the soil profile. With the BIOLOG system we observed the general trend that the capability of utilizing diverse Substrates decreased with soil depth whereas a few Substrates, such as Tween 40 and Tween 80 could be utilised by the bacteria of all soil depths. (C) 2005 Elsevier B.V. All rights reserved.