Regulation of expression of the Epstein-Barr virus BamHI-A rightward transcripts
Regulation of expression of the Epstein-Barr virus BamHI-A rightward transcripts
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DOI：
10.1128/jvi.79.3.1724-1733.2005
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发表时间：
2005-02-01
影响因子：
5.4
通讯作者：
Hayward, SD
中科院分区：
文献类型：
--
作者：
Chen, HL;Huang, I;Hayward, SD
EB病毒（EBV）BamHI-A转录物或BART是在所有EBV潜伏期程序中表达的mRNA家族，包括健康携带者中的EBV感染的B细胞。尽管它们普遍存在，但BARTs的调控和生物学功能仍不清楚。在该研究中，通过使用选择加帽的全长mRNA的程序来表征BART 5'末端。两个TATA少启动子区，指定P1和P2，进行了定位。PI在上皮细胞和B细胞中具有相对较高的基础活性，而P2在上皮细胞中表现出较高的活性。在EBV感染B细胞后，感染后不久检测到P1的转录，而P2的表达被延迟。瞬时转染细胞中的启动子-报告基因分析显示，P1和P2的差异调节。干扰素调节因子7（IRF 7）和IRF 5负调节P1活性。c-Myc和C/EBP家族成员正调控P2。通过电泳迁移率变动分析、染色质免疫沉淀和报告基因分析来表征C/EBP对P2的调节。上皮细胞中更丰富的BART表达与这些细胞中阳性和阴性调节因子的相对表达相关。
The Epstein-Barr virus (EBV) BamHI-A rightward transcripts, or BARTs, are a family of mRNAs expressed in all EBV latency programs, including EBV-infected B cells in healthy carriers. Despite their ubiquitous expression, the regulation and biological function of BARTs are still unclear. In this study, the BART 5' termini were characterized by using a procedure that selects capped, full-length mRNAs. Two TATA-less promoter regions, designated P1 and P2, were mapped. PI had relatively high basal activity in both epithelial and B cells, whereas P2 exhibited higher activity in epithelial cells. Upon EBV infection of B cells, transcription from PI was detected soon after infection, while expression from P2 was delayed. Promoter-reporter assays in transiently transfected cells revealed that P1 and P2 were differentially regulated. Interferon regulatory factor 7 (IRF7) and IRF5 negatively regulated P1 activity. c-Myc and C/EBP family members positively regulated P2. Regulation of P2 by C/EBPs was characterized by electrophoretic mobility shift assay, chromatin immunoprecipitation, and reporter assays. More-abundant BART expression in epithelial cells correlated with the relative expression of positive and negative regulators in these cells.