Time course of aquaporin expression after transient focal cerebral ischemia in mice
Time course of aquaporin expression after transient focal cerebral ischemia in mice
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DOI：
10.1002/jnr.20819
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发表时间：
2006-05-15
影响因子：
4.2
通讯作者：
Badaut, Jerome
中科院分区：
文献类型：
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作者：
Ribeiro, Marlise de Castro;Hirt, Lorenz;Badaut, Jerome
脑水肿有助于中风的发病率和死亡率。水通道蛋白（AQP）-1、-4和-9被认为是脑内三种主要的水通道。为了阐明它们在水运动中的作用，我们将它们的表达模式与短暂局灶性脑缺血后的脑肿胀进行了比较。脑缺血后1小时和48小时出现两个最大半球肿胀峰，与AQP 4表达的两个高峰相吻合。在闭塞后1小时，AQP 4的表达显着增加的星形胶质细胞终足的核心和边界的病变。在48小时，AQP 4的表达增加，星形胶质细胞在整个细胞的病变边缘。AQP 9在24小时显示出显著的诱导，其随着时间逐渐增加，与肿胀无关。AQP 1的表达无明显变化。这些结果表明，AQP 4，而不是AQP 1或AQP 9，可能发挥重要作用的水运动与小鼠短暂性脑缺血后水肿的病理生理学。(c)2006 Wiley-Liss，Inc.
Cerebral edema contributes to morbidity and mortality in stroke. Aquaporins (AQPs)-1, -4, and -9 have been identified as the three main water channels in the brain. To clarify their role in water movement, we have compared their expression patterns with brain swelling after transient focal brain ischemia. There were two peaks of maximal hemispheric swelling at 1 hr and at 48 hr after ischemia, coinciding with two peaks of AQP4 expression. At 1 hr after occlusion, AQP4 expression was significantly increased on astrocyte endfeet in the core and in the border of the lesion. At 48 hr, AQP4 expression was increased in astrocytes in the border of the lesion over the whole cell. AQP9 showed a significant induction at 24 hr that increased gradually with time, without correlation with the swelling. The expression of AQP1 remained unchanged. These results suggest that AQP4, but not AQP1 or AQP9, may play an important role in water movement associated with the pathophysiology of edema after transient cerebral ischemia in the mouse. (c) 2006 Wiley-Liss, Inc.